MP11-19 MAGNETIC RESONANCE VISIBILITY OF IRON LABELED MESENCHYMAL STEM CELLS FOR ENDOSCOPIC INJECTION INTO THE PORCINE URETHRAL SPHINCTER
MP11-19 MAGNETIC RESONANCE VISIBILITY OF IRON LABELED MESENCHYMAL STEM CELLS FOR ENDOSCOPIC INJECTION INTO THE PORCINE URETHRAL SPHINCTER
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MP11-19 内镜注射猪尿道括约肌的铁标记间充质干细胞的磁共振可见度
DOI：
10.1016/j.juro.2015.02.400
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kramer U
中科院分区：
文献类型：
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作者：
Will S;Sievert K.D;Vaegler M;Eibofner F;Martirosian P;Schick F;Schmehl J;Grözinger G;Bantleon R;Nikolaou K;Kramer U
方法MSC 用 SPIO 颗粒标记，用于分析迁移和活力。进行体模 MR 测量以评估标记效果。对于短期和长期研究，铁标记的 MSC 经内窥镜注射到猪尿道括约肌中，并在 3T MRI 扫描仪上进行监测。 MSC注射后三周、三个月和六个月对动物进行检查。使用快速自旋回波序列测量尿道括约肌的高分辨率解剖图像来检测 SPIO 颗粒。利用具有多个回波时间的梯度回波序列来视觉识别和验证铁颗粒的位置。然后将注射细胞的分布与组织学结果相关联（见图）。结果铁标记后细胞参数的功能分析显示对细胞的增殖和分化没有影响。随着铁剂量的增加，粘附性增加，迁移能力下降，可能是铁标记的影响。
METHODSMSC were labeled with SPIO particles for analysis of migration and viability. Phantom MR measurements were performed in order to evaluate labeling effects. For short and long term studies, iron labeled MSCs were endoscopically injected into the porcine urethral sphincter and monitored on a 3T MRI scanner. The animals were examined three weeks, three and six months after MSC injection. High resolution anatomical images of the urethral sphincter were measured to detect SPIO particles using a fast-spin-echo sequence. A gradient echo sequence with multiple echo times was utilized to visually identify and verify locations of iron particles. The distribution of injected cells was then correlated with histological findings (see Figure).RESULTSFunctional analysis of cell parameters after iron labeling showed no effects on proliferation and differentiation of the cells. The adherence increased with a higher iron dose, and the ability to migrate decreased presumably the effect of iron labeling.