Coronin 2B regulates dendrite outgrowth by modulating actin dynamics
Coronin 2B regulates dendrite outgrowth by modulating actin dynamics
复制标题
Coronin 2B 通过调节肌动蛋白动力学来调节树突生长
DOI：
10.1002/1873-3468.13886
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发表时间：
2020-07-31
期刊：
影响因子：
3.5
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Chen, Yuewen;Xu, Jinying;Chen, Yu
细胞骨架重塑对于神经元结构和功能的发育和维持是必不可少的。然而，在这些过程中，控制肌动蛋白聚合和解聚之间平衡的分子机制并不完全清楚。在这里，我们报告说，冠状蛋白2B，一种保守的肌动蛋白结合蛋白，集中在发育树突的尖端，冠状蛋白2B的敲除抑制树突的生长。重要的是，冠蛋白2B与肌动蛋白相互作用，降低F-肌动蛋白/G-肌动蛋白的比例。此外，冠蛋白2B的卷曲螺旋结构域是其寡聚化所必需的，因此将冠蛋白2B限制在神经突尖端。我们的研究结果共同表明，冠状蛋白2B是重要的，通过限制生长锥肌动蛋白聚合的速度，促进树突生长。
Cytoskeletal remodeling is indispensable for the development and maintenance of neuronal structures and functions. However, the molecular machinery that controls the balance between actin polymerization and depolymerization during these processes is incompletely understood. Here, we report that coronin 2B, a conserved actin‐binding protein, is concentrated at the tips of developing dendrites and that knockdown of coronin 2B inhibits the growth of dendrites. Importantly, coronin 2B interacts with actin and reduces the F‐actin/G‐actin ratio. Furthermore, the coiled‐coil domain of coronin 2B is required for its oligomerization, thus confining coronin 2B to neurite tips. Our findings collectively suggest that coronin 2B is important for promoting dendrite outgrowth by limiting the speed of actin polymerization at growth cones.