Free-living nematodes associated with pine cones of Pinus thunbergii and P. taeda at Japanese coastal and inland forest sites
Free-living nematodes associated with pine cones of Pinus thunbergii and P. taeda at Japanese coastal and inland forest sites
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DOI：
10.1163/15685411-00003221
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发表时间：
2019
期刊：
影响因子：
1.2
通讯作者：
Y. Kitagami;N. Kanzaki;T. Tanikawa;Y. Matsuda
中科院分区：
文献类型：
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作者：
Y. Kitagami;N. Kanzaki;T. Tanikawa;Y. Matsuda
我们调查了在三个沿海和内陆栖息地的56个从活树枝和森林地面上采集的黑松球果和在不同高度采集的11个火炬松球果中线虫的分布。通过18S小亚基rRNA基因序列分析，我们鉴定了47种线虫的科或属。自由生活的球果线虫在沿海黑松、内陆黑松和火炬松中的出现频率分别为97%、92%和82%。系统发育分析分配的线虫到四个分支具有较高的自举值。九个序列，只发现在锥上活的分支与Panagrobelus stammeri和一个未知的Panagrobelus属聚在一起。我们的研究结果表明，线虫通常与松树林生态系统中的锥，脱水能力可能是地上生存的关键。
We surveyed the distribution of nematodes in 56 cones of Pinus thunbergii collected from both live branches and on the forest floor in three coastal and inland habitats and in 11 cones of P. taeda collected at different heights. We identified 47 nematodes to family or genera by analysis of an 18S small subunit rRNA gene sequence. The frequencies of occurrence of free-living cone nematodes were 97% in coastal P. thunbergii, 92% in inland P. thunbergii, and 82% in P. taeda. Phylogenetic analysis assigned the nematodes to four clades with high bootstrap values. Nine sequences that were found only in cones on live branches were clustered with Panagrobelus stammeri and an unknown Panagrobelus sp. Our results imply that nematodes are commonly associated with cones in pine forest ecosystems and that a capacity for anhydrobiosis may be a key to surviving above-ground.