Involvement of TRPM2 and TRPM8 in temperature-dependent masking behavior
Involvement of TRPM2 and TRPM8 in temperature-dependent masking behavior
复制标题
TRPM2 和 TRPM8 参与温度依赖性掩蔽行为
DOI：
10.1038/s41598-019-40067-x
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发表时间：
2019
影响因子：
4.6
通讯作者：
Yoshimura T
中科院分区：
文献类型：
--
作者：
Ota W;Nakane Y;Kashio M;Suzuki Y;Nakamura K;Mori Y;Tominaga M;Yoshimura T
掩蔽是对环境变化的直接行为反应，在活动的时间分布中起着重要作用。然而，负责掩蔽的机制仍不清楚。在这里，我们确定了温度传感器和一个可能的神经回路调节小鼠的温度依赖性掩蔽行为。对缺乏热敏瞬时受体电位（TRP）通道（Trpv 1/3/4和Trpm 2/8）的小鼠的分析表明，在Trpm 2和Trpm 8缺失的小鼠中，温度依赖性掩蔽受损。在温度依赖性掩蔽期间，几个大脑区域被激活，包括视前区（POA），被称为体温调节中心，视交叉上核（SCN），这是主要的昼夜节律起搏器，丘脑室旁核（PVT）和丘脑核（NAc）。POA、SCN、PVT是相互关联的，PVT向NAc发送密集的投射，NAc是参与车轮运行活动的关键大脑区域。部分化学病变的PVT减弱掩蔽，这表明参与的PVT温度依赖性掩蔽行为。
Masking is a direct behavioral response to environmental changes and plays an important role in the temporal distribution of activity. However, the mechanisms responsible for masking remain unclear. Here we identify thermosensors and a possible neural circuit regulating temperature-dependent masking behavior in mice. Analysis of mice lacking thermosensitive transient receptor potential (TRP) channels (Trpv1/3/4andTrpm2/8) reveals that temperature-dependent masking is impaired inTrpm2- andTrpm8-null mice. Several brain regions are activated during temperature-dependent masking, including the preoptic area (POA), known as the thermoregulatory center, the suprachiasmatic nucleus (SCN), which is the primary circadian pacemaker, the paraventricular nucleus of the thalamus (PVT), and the nucleus accumbens (NAc). The POA, SCN, PVT are interconnected, and the PVT sends dense projections to the NAc, a key brain region involved in wheel-running activity. Partial chemical lesion of the PVT attenuates masking, suggesting the involvement of the PVT in temperature-dependent masking behavior.