IMMUNOCYTOCHEMICAL LOCALIZATION OF ESTROGEN-INDUCED PROGESTIN RECEPTORS IN GUINEA-PIG BRAIN
IMMUNOCYTOCHEMICAL LOCALIZATION OF ESTROGEN-INDUCED PROGESTIN RECEPTORS IN GUINEA-PIG BRAIN
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DOI：
10.1016/0006-8993(88)90664-6
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发表时间：
1988-11-22
期刊：
影响因子：
2.9
通讯作者：
TURCOTTE, J
中科院分区：
文献类型：
--
作者：
BLAUSTEIN, JD;KING, JC;TURCOTTE, J
用抗孕激素受体的单抗和双桥过氧化物酶-抗过氧化物酶技术相结合，建立了一种灵敏的免疫细胞化学方法，显示雌激素诱导的豚鼠脑内孕激素受体免疫反应(PR-IR)。PR-IR神经元分布于下丘脑和视前区。在弓状核、室旁视前区、视前内侧核、视前内侧区和下丘脑腹外侧区中阳性细胞最多。PR-IR阳性细胞在终纹床核、室旁核和下丘脑外侧区较少，散在分布于下丘脑和视前区。PR-IR主要分布在核内，神经元突起染色较浅。电子显微镜证实了反应产物主要分布在核内，并进一步证明反应产物分散在细胞核内，但与核仁无关。在无雌二醇刺激的情况下，仅观察到少量PR-IR细胞，且反应产物比有雌二醇时轻得多。注射孕酮对PR-IR强度无明显影响。
By using a combination of monoclonal antibodies to progestin receptors and a double-bridge peroxidase-antiperoxidase technique, a sensitive immunocytochemical method was developed for visualizing progestin receptor immunoreactively (PR-IR) in brains of estrogen-primed guinea pigs. PR-IR neurons were observed throughout the hypothalamus and preoptic area. They were seen in largest numbers in the arcuate nucleus, periventricular preoptic regions, medial preoptic nucleus, medial preoptic area and in the ventrolateral area of the hypothalamus. Lower numbers of PR-IR positive cells were detected in the bed nucleus of stria terminalis, paraventricular nucleus and lateral hypothalamus with scattered cells seen throughout the hypothalamus and preoptic area. The PR-IR was mostly intranuclear with lighter staining in neuronal processes. Electron microscopy confirmed the predominantly intranuclear localization and further demonstrated that the reaction product was dispersed throughout the nucleus, but not associated with the nucleolus. Few PR-IR cells were observed in the absence of estradiol priming, and the reaction product was much lighter than in the presence of estradiol. Progesterone injection was without apparent effect on intensity of the PR-IR.