Effect of Prostaglandin E 2 on Human Dental Follicle Cells during Osteogenic Differentiation
Effect of Prostaglandin E 2 on Human Dental Follicle Cells during Osteogenic Differentiation
复制标题
前列腺素E 2 对人牙囊细胞成骨分化的影响
DOI：
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
N. Ogura
中科院分区：
文献类型：
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作者：
Hidesuke Yoshimoto;N. Ogura
人牙囊干/祖细胞分化为成骨细胞。为了研究与人牙囊细胞（hDFCs）成骨分化/矿化相关的因素，我们进行了hDFCs成骨分化过程中的基因表达谱分析。与生长培养基（GM）相比，在成骨诱导培养基（OIM）中培养的hDFC中环氧合酶（考克斯）-1和-2上调。前列腺素E2（PGE 2）是研究最多的通过COX作用从花生四烯酸衍生的前列腺素类，可调节骨代谢。所有PGE 2 E型前列腺素受体（EP），EP 1-EP 4，在hDFC中表达。Real-time PCR结果显示，与第0天相比，在OIM和GM中培养的hDFCs在第10天和第17天的EP 2和EP 4的表达增加。我们使用hDFCs研究了PGE 2在成骨分化中的作用。PGE 2降低osterix和碱性磷酸酶的基因表达水平，这是与成骨分化相关的因素。茜素红S染色显示PGE 2对hDFCs的基质矿化有抑制作用。这些发现表明PGE 2可能抑制干细胞/祖细胞的成骨分化/矿化。
Stem/progenitor cells isolated from human dental follicles differentiate into osteogenic cells. To investigate factors associated with osteogenic differentiation/mineralization in human dental follicle cells(hDFCs), we performed gene expression profiling of hDFCs during osteogenic differentiation. Cyclooxygenase(COX)-1 and -2 were up-regulated in hDFCs cultured in osteogenic induction medium(OIM)compared to growth medium(GM). Prostaglandin E2(PGE2), which is the most studied prostanoid derived from arachidonic acid through the actions of COXs, may regulate bone metabolism. All PGE2 E-type prostanoid receptors(EP), EP1-EP4, were expressed in hDFCs. Real-time PCR showed that the expression of EP2 and EP4 was increased in hDFCs cultured in OIM and GM at days 10 and 17 compared to day 0. We investigated the action of PGE2 in osteogenic differentiation using hDFCs. PGE2 decreased gene expression levels of osterix and alkaline phosphatase, which are factors associated with osteogenic differentiation. PGE2 elicited inhibitory action on matrix mineralization of hDFCs as seen with alizarin red S staining. These findings suggest that PGE2 may inhibit osteogenic differentiation/mineralization of stem/progenitor cells.
影响因子：
4.6
作者：
Yang Chai;Xiaobing Jiang;Yoshihiro Ito;P. Bringas;Jun Han;David H. Rowitch;Philippe Soriano
通讯作者：
Yang Chai;Xiaobing Jiang;Yoshihiro Ito;P. Bringas;Jun Han;David H. Rowitch;Philippe Soriano
DOI：
10.1016/j.prostaglandins.2008.02.001
发表时间：
2008-06-01
影响因子：
2.9
作者：
Choudhary, Shilpa;Alander, Cynthia;Raisz, Lawrence
通讯作者：
Raisz, Lawrence