Tissue macroarray: A simple and cost-effective method for high-throughput studies
Tissue macroarray: A simple and cost-effective method for high-throughput studies
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DOI：
10.1097/00129039-200306000-00015
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发表时间：
2003-06-01
影响因子：
1.6
通讯作者：
Liu, JS
中科院分区：
文献类型：
--
作者：
Wang, L;Deavers, MT;Liu, JS
组织微阵列技术是一种强大的技术，可以在一张载玻片上检查许多临床标本。然而，该技术需要专门的仪器来构建组织微阵列块。作者描述了一种替代组织宏阵列技术，用于在单个载玻片上同时分析多个临床标本。作为这种方法的一个例子，作者将来自12个不同石蜡块的12个肿瘤样本组装在载玻片上，然后用hMLH1抗体进行免疫染色。这种简单的方法不需要专门的设备，应该是一种替代的大规模临床标本的有效方法。
The tissue microarray technique is a powerful technique that can examine many clinical specimens in a single slide. However, this technique requires a specialized instrument for the construction of tissue microarray block. The authors describe an alternative tissue macroarray technique for the simultaneous analysis of multiple clinical specimens on a single slide. As an example of this method, the authors assembled 12 tumor samples from 12 different paraffin blocks on a glass slide and then immunostained with hMLH1 antibody. This simple method does not need specialized equipment and should be an alternative efficient approach for large-scale clinical specimens.