Lipidomic Profiling of Human Milk Derived Exosomes and Their Emerging Roles in the Prevention of Necrotizing Enterocolitis
Lipidomic Profiling of Human Milk Derived Exosomes and Their Emerging Roles in the Prevention of Necrotizing Enterocolitis
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人乳源性外泌体的脂质组学分析及其在预防坏死性小肠结肠炎中的新作用
DOI：
10.1002/mnfr.202000845
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发表时间：
2021-04-22
影响因子：
5.2
通讯作者：
Han, Shuping
中科院分区：
文献类型：
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作者：
Chen, Wenjuan;Chen, Xiaohui;Han, Shuping
范围：母乳可以预防坏死性小肠结肠炎（NEC）的发展。人乳富含参与细胞间通讯的携带货物的外泌体。本研究调查的影响，长期和早产的人乳源性外泌体，并阐明其脂质expression profiles.Methods和结果：从健康的母亲收集谁交付足月或早产儿的牛奶，外泌体分离和量化。足月和早产乳外泌体的给药在体外显著增强上皮细胞增殖和迁移，并在体内改善NEC的严重程度。在足月和早产人乳源性外泌体中共鉴定出395种脂质。生物信息学分析和蛋白质印迹法显示，前50种脂质通过细胞外信号调节激酶/丝裂原活化蛋白激酶（ERK/MAPK）途径调节肠上皮细胞功能。结论：本研究首次揭示了早产儿和足月乳来源的外泌体中脂质组学的复杂性。这些结果为母乳如何预防NEC的发展提供了新的机制见解。
Scope: Human milk can prevent the development of necrotizing enterocolitis (NEC). Human milk is rich in cargo-carrying exosomes that participate in intercellular communication. This study investigated the effects of term and preterm human milk-derived exosomes, and elucidated their lipid expression profiles.Methods and Results: Milk from healthy mothers is collected who have delivered full-term or preterm infants, and exosomes are isolated and quantified. Administration of term and preterm milk exosomes significantly enhances epithelial proliferation and migration in vitro, and ameliorates the severity of NEC in vivo. A total of 395 lipids are identified in term and preterm human milk-derived exosomes. Bioinformatics analysis and western blotting reveal that top 50 lipids regulate intestinal epithelial cell function via the Extracellular-Signal-Regulated Kinase/Mitogen Activated Protein Kinase (ERK/MAPK) pathway.Conclusion: This study reveals for the first time the lipidomic complexities in exosomes derived from preterm and term milk. The results provide novel mechanistic insight on how human milk prevents the development of NEC.