Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila.
Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila.
复制标题
果蝇中饮食中的无眼无眼蘑菇体神经细胞增殖。
DOI：
10.7554/elife.26343
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发表时间：
2017-08-09
期刊：
影响因子：
7.7
通讯作者：
Siegrist SE
中科院分区：
文献类型：
--
作者：
Sipe CW;Siegrist SE
细胞增殖与营养物质的可用性相结合。如果营养物质变得有限，增殖停止，因为生长因子和/或PI 3-激酶活性水平减弱。在这里，我们报告一个例外，这种普遍性内的果蝇神经干细胞（神经母细胞）的亚群。我们发现大多数神经母细胞以营养依赖的方式进入和退出细胞周期，这种方式是可逆的，并受PI 3-激酶的调节。然而，一个小的子集，蘑菇体神经母细胞，产生重要的记忆和学习的神经元，分裂独立的饮食营养条件和PI 3激酶活性。这种不依赖于营养的增殖是由Eyeless调节的，Eyeless是Pax-6的直系同源物，在蘑菇体成神经细胞中表达。当Eyeless被击倒时，蘑菇体神经母细胞在营养物质被撤回时退出细胞周期。相反，当Eyeless异位表达时，一些非蘑菇体神经母细胞独立于饮食营养条件而分裂。因此，Eyeless将MB成神经细胞增殖与营养物质的可用性分离，从而在营养物质缺乏的条件下允许脑亚区域中的优先神经发生。
Cell proliferation is coupled with nutrient availability. If nutrients become limited, proliferation ceases, because growth factor and/or PI3-kinase activity levels become attenuated. Here, we report an exception to this generality within a subpopulation of Drosophila neural stem cells (neuroblasts). We find that most neuroblasts enter and exit cell cycle in a nutrient-dependent manner that is reversible and regulated by PI3-kinase. However, a small subset, the mushroom body neuroblasts, which generate neurons important for memory and learning, divide independent of dietary nutrient conditions and PI3-kinase activity. This nutrient-independent proliferation is regulated by Eyeless, a Pax-6 orthologue, expressed in mushroom body neuroblasts. When Eyeless is knocked down, mushroom body neuroblasts exit cell cycle when nutrients are withdrawn. Conversely, when Eyeless is ectopically expressed, some non-mushroom body neuroblasts divide independent of dietary nutrient conditions. Therefore, Eyeless uncouples MB neuroblast proliferation from nutrient availability, allowing preferential neurogenesis in brain subregions during nutrient poor conditions.