STAC-A New Domain Associated with Transmembrane Solute Transport and Two-Component Signal Transduction Systems
STAC-A New Domain Associated with Transmembrane Solute Transport and Two-Component Signal Transduction Systems
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DOI：
10.1016/j.jmb.2015.08.017
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发表时间：
2015-10-09
影响因子：
5.6
通讯作者：
Lupas, Andrei N.
中科院分区：
文献类型：
--
作者：
Korycinski, Mateusz;Albrecht, Reinhard;Lupas, Andrei N.
跨膜受体是原核生物感觉通路的组成部分。这些受体具有共同的二聚体结构，其基本形式包括N-末端细胞外传感器、跨膜螺旋和细胞内效应器。作为一个例外，我们已经确定了一个古生菌受体家族的例子Af 1503从闪烁古生菌，这是C-末端缩短，缺乏一个可识别的效应模块。相反，HAMP结构域形成细胞溶质中信号转导的唯一延伸。在这里，我们研究的基因环境的Af 1503样受体，并发现一个频繁的关联与跨膜转运蛋白。此外，我们确定并定义了一个密切相关的新的蛋白质结构域家族，我们使用Af 1502从A.闪电。该家族的成员既作为独立的蛋白质也作为现存受体内的结构域被发现。一般而言，后者作为溶质载体5（SLC 5）样跨膜结构域和双组分信号转导（TCST）结构域之间的连接体出现。例如，这在组氨酸激酶CbrA中可见，其是假单胞菌中代谢、毒力和抗生素抗性的全局调节剂。我们建议，这个新确定的结构域家族介导的信号转导系统调节运输过程，并命名为STAC，SLC和TCST相关组件。(c)2015 MRC分子生物学实验室。由爱思唯尔有限公司发布
Transmembrane receptors are integral components of sensory pathways in prokaryotes. These receptors share a common dimeric architecture, consisting in its basic form of an N-terminal extracellular sensor, transmembrane helices, and an intracellular effector. As an exception, we have identified an archaeal receptor family-exemplified by Af1503 from Archaeoglobus fulgidus-that is C-terminally shortened, lacking a recognizable effector module. Instead, a HAMP domain forms the sole extension for signal transduction in the cytosol. Here, we examine the gene environment of Af1503-like receptors and find a frequent association with transmembrane transport proteins. Furthermore, we identify and define a closely associated new protein domain family, which we characterize structurally using Af 1502 from A. fulgidus. Members of this family are found both as stand-alone proteins and as domains within extant receptors. In general, the latter appear as connectors between the solute carrier 5 (SLC5) like transmembrane domains and two-component signal transduction (TCST) domains. This is seen, for example, in the histidine kinase CbrA, which is a global regulator of metabolism, virulence, and antibiotic resistance in Pseudomonads. We propose that this newly identified domain family mediates signal transduction in systems regulating transport processes and name it STAC, for SLC and TCST-Associated Component. (c) 2015 MRC Laboratory of Molecular Biology. Published by Elsevier Ltd.