Use of a standard strain for external calibration in behavioral phenotyping
Use of a standard strain for external calibration in behavioral phenotyping
复制标题
DOI：
10.1007/s10519-005-3224-1
复制
发表时间：
2005-05-01
影响因子：
2.6
通讯作者：
Makino, J
中科院分区：
文献类型：
--
作者：
Blizard, DA;Wada, Y;Makino, J
本文评价了在多菌株行为表型分析方案中纳入标准菌株或远系繁殖原种，以在生化测定程序中发挥与外标相同的作用。作为潜在的标准，F344近交系和远交系的龙埃文斯股票进行了测试与其他三个近交系。为了评估饲养条件对表型稳定性的影响，一组F344在筑波大学出生，另一组在其他地方繁殖并在4周龄时送到筑波。所有动物作为成年动物进行旷场（OF）、跑道出现（RE）和挖掘试验。结果表明，饲养史和运输史对两个F344群体的OF和RE行为没有影响，但有证据表明，不同的饲养经历可能会影响挖掘行为。在涉及大鼠、小鼠和苍蝇的多个近交系或品系的表型鉴定方案中纳入标准品系或原种具有明显的优势，可为实验室间比较提供参考点。属性的近交系和远交股票有利于他们作为标准使用进行了讨论。
The present paper evaluates the inclusion of a standard strain or outbred stock in multi-strain behavioral phenotyping protocols to perform the same role as the external standard in biochemical assay procedures. As potential standards, the F344 inbred strain and an outbred stock of Long Evans were tested with three other inbred strains. To evaluate the influence of rearing conditions on phenotype stability, one group of F344s was born at the University of Tsukuba, another, bred elsewhere and delivered to Tsukuba at 4 weeks of age. All animals were tested in open-field (OF), runway emergence (RE) and digging tests as adults. The results showed no influence of breeding or transportation history on OF and RE behavior of the two F344 groups, while there was evidence that digging behavior may be affected by the different rearing experience. The inclusion of a standard strain or stock in phenotyping protocols involving multiple inbred strains or lines of rats, mice and flies has obvious advantages by providing a reference point for inter-laboratory comparisons. The properties of inbred strains and outbred stocks favorable to their use as standards are discussed.