PROTECTIVE EFFECT OF 2,2′-DITHIENYL DISELENIDE ON KAINIC ACID-INDUCED NEUROTOXICITY IN RAT HIPPOCAMPUS
PROTECTIVE EFFECT OF 2,2′-DITHIENYL DISELENIDE ON KAINIC ACID-INDUCED NEUROTOXICITY IN RAT HIPPOCAMPUS
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DOI：
10.1016/j.neuroscience.2011.07.038
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发表时间：
2011-10-13
期刊：
影响因子：
3.3
通讯作者：
Nogueira, C. W.
中科院分区：
文献类型：
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作者：
Bortolatto, C. F.;Jesse, C. R.;Nogueira, C. W.
在这项研究中，我们研究了 2,2'-二噻吩基二硒化物 (DTDS)（一种有机硒化合物）对红藻氨酸 (KA) 诱导的大鼠癫痫发作的作用。在注射 KA（10 mg/kg，腹腔注射）前 1 小时，通过口服途径对大鼠进行 DTDS（50 或 100 mg/kg）预处理。我们的结果表明，DTDS (100 mg/kg) 可有效延长 KA 诱发的第一次阵挛性癫痫发作的潜伏期，并减少癫痫发作和拉辛评分。 DTDS 还导致大鼠海马和大脑皮层中 KA 暴露导致的兴奋性脑电图 (EEG) 变化减少。此外，DTDS（50 和 100 mg/kg）改善了用 KA 治疗的大鼠海马中升高的反应性物种 (RS) 和羰基蛋白水平以及 Na+、K+-ATP 酶活性。最后，甲酚紫染色证明，注射 KA 7 天后，DTDS (100 mg/kg) 对大鼠海马中 KA 诱导的神经变性具有保护作用。总之，本研究表明 DTDS 可以减轻 KA 诱导的大鼠癫痫持续状态以及随后的海马损伤。 (C) 2011 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
In this study, we investigated the effects of 2,2'-dithienyl diselenide (DTDS), an organoselenium compound, against seizures induced by kainic acid (KA) in rats. Rats were pretreated with DTDS (50 or 100 mg/kg) by oral route 1 h before KA injection (10 mg/kg, intraperitoneal). Our results showed that DTDS (100 mg/kg) was effective in increasing latency for the onset of the first clonic seizure episode induced by KA, as well as in decreasing the appearance of seizures and the Racine's score. DTDS also caused a decrease in the excitatory electroencephalographic (EEG) changes, resulting from KA exposure in hippocampus and cerebral cortex of rats. Besides, elevated reactive species (RS) and carbonyl protein levels and Na+, K+-ATPase activity in hippocampus of rats treated with KA were ameliorated by DTDS (50 and 100 mg/kg). Lastly, as evidenced by Cresyl-Violet stain, DTDS (100 mg/kg) elicited a protective effect against KA-induced neurodegeneration in rat hippocampus 7 days after KA injection. In conclusion, the present study showed that DTDS attenuated KA-induced status epilepticus in rats and the subsequent hippocampal damage. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.