Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses.
Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses.
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DOI：
10.1103/physrevlett.119.158102
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发表时间：
2017-10-13
影响因子：
8.6
通讯作者：
Mancuso AP
中科院分区：
文献类型：
--
作者：
Kurta RP;Donatelli JJ;Yoon CH;Berntsen P;Bielecki J;Daurer BJ;DeMirci H;Fromme P;Hantke MF;Maia FRNC;Munke A;Nettelblad C;Pande K;Reddy HKN;Sellberg JA;Sierra RG;Svenda M;van der Schot G;Vartanyants IA;Williams GJ;Xavier PL;Aquila A;Zwart PH;Mancuso AP
我们使用来自x射线自由电子激光(XFEL)的极其明亮和超短的脉冲来测量从单个生物物品散射的x射线的相关性。这使我们能够超越传统的结晶学和单粒子成像方法来研究结构。我们使用角关联从直线加速器相干光源测量的X射线衍射数据中恢复纳米级病毒的三维(3D)结构。相关性为我们提供了3D病毒的全面结构指纹，我们将其用于基于模型的结构恢复和从头计算结构恢复。分析表明，病毒的结构偏离了预期的完美二十面体对称性。我们的结果为XFEL通过角度关联研究纳米级物体的结构和动力学提供了令人兴奋的机会。
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.