Homologs of the sexually induced gene 1 (sig1) product constitute the stramenopile mastigonemes
Homologs of the sexually induced gene 1 (sig1) product constitute the stramenopile mastigonemes
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DOI：
10.1016/j.protis.2006.08.004
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发表时间：
2007-01-01
期刊：
影响因子：
2.5
通讯作者：
Murakami, Akio
中科院分区：
文献类型：
--
作者：
Honda, Daiske;Shono, Takanori;Murakami, Akio
前鞭毛上的三分体管状鞭毛体是层毛的一个形态特征。无论从系统学还是细胞生物学的角度来看，鞭毛体都是重要的和潜在的信息结构。然而，很少有生化研究已报告的stratimile mastigonemes。利用SIDS-PAGE和蛋白质测序技术成功地分离和分析了丝管藻的鞭毛。一个鞭毛蛋白（115 kDa）的部分氨基酸序列显示出与中心硅藻的性诱导基因1（sig 1）的产物高度相似。针对115-kDa的蛋白质的多克隆抗体反应不仅在Scytosiphon lomentaria的鞭毛体的轴，但也有另一个明显不同的层鞭毛虫，Sulcochrysis biplastida（Dictyochophycae）。因此，我们建议，115 kDa的蛋白质（即Sig 1同源物）是管状轴的鞭毛体的组成部分。(c)2006年Elsevier GmbH。All rights reserved.
The tripartite tubular mastigoneme on the anterior flagellum is a morphological feature that characterizes the stramenopiles. Mastigonemes are significant and potentially informative structures not only from the viewpoint of systematics, but also of cell biology. Nevertheless, few biochemical studies have been reported on stramenopile mastigonemes. The flagella of Scytosiphon lomentaria (Phaeophyceae) were successfully isolated and analyzed using SIDS-PAGE followed by protein sequencing. The partial amino acid sequence of one flagellar protein (115 kDa) showed high similarity with the sexually induced gene 1 (sig1) product of centric diatoms. A polyclonal antibody against the 115-kDa protein reacted not only to the shaft of mastigonemes in Scytosiphon lomentaria, but also another distinctly different stramenopile flagellate, Sulcochrysis biplastida (Dictyochophyceae). Therefore, we propose that the 115-kDa protein (i.e. Sig1 homologs) is a constituent of the tubular shaft of the mastigoneme. (c) 2006 Elsevier GmbH. All rights reserved.