Histology and research at the hard tissue-implant interface using Technovit 9100 New embedding technique
Histology and research at the hard tissue-implant interface using Technovit 9100 New embedding technique
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DOI：
10.1016/j.actbio.2010.06.022
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发表时间：
2010-11-01
影响因子：
9.7
通讯作者：
Witte, Frank
中科院分区：
文献类型：
--
作者：
Willbold, Elmar;Witte, Frank
钙化组织，如骨骼和牙齿，是组织学研究中最具挑战性的组织之一。然而，特别是在牙科或整形外科研究方面，强大的组织学技术对于研究病理条件或创伤性损伤以及研究再生过程和功能恢复的分子和细胞机制是必要的。在骨科研究中，情况甚至更加复杂，因为这里经常存在金属植入物或由各种材料制成的其他装置，并且硬组织-植入物界面在生物相容性和功能恢复研究中具有至关重要的意义。在切割-研磨技术之后，嵌入技术树脂是最有前途的方法。在这里，我们描述了一种使用Technovit 9100 New的优化和标准化的嵌入和切割技术。使用这种技术，我们能够进行酶组织化学，免疫组织化学，各种经典的组织学染色，甚至原位杂交。(C)2010 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Calcified tissues, like bones and teeth, are among the most challenging tissues for histological research. However, especially with respect to dental or orthopaedic research, powerful histological techniques are necessary to study pathological conditions or traumatic injuries, and to investigate the molecular and cellular mechanisms of regeneration processes and functional recovery. The situation is even more complicated in orthopaedic research because here metallic implants or other devices made of various materials are often present, and the hard tissue-implant interface is of crucial interest in both biocompatibility and functional recovery research. After the cutting-grinding technique, embedding in technical resins is the most promising approach. Here we describe an optimized and standardized embedding and cutting technique using Technovit 9100 New. Using this technique, we are able to perform enzyme histochemistry, immunohistochemistry, a great variety of classical histological stains and even in situ hybridization. (C) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.