DYSMYELINATION OF SHIVERER MUTANT MICE INVIVO AND INVITRO
DYSMYELINATION OF SHIVERER MUTANT MICE INVIVO AND INVITRO
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DOI：
10.1111/j.1471-4159.1982.tb12560.x
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发表时间：
1982-01-01
影响因子：
4.7
通讯作者：
TSUKADA, Y
中科院分区：
文献类型：
--
作者：
NAGAIKE, K;MIKOSHIBA, K;TSUKADA, Y
在体内和体外研究了颤抖突变小鼠中枢神经系统的髓鞘形成障碍。采用胶质纤维酸性蛋白抗体进行免疫组织化学反应，在颤抖者小脑白质中观察到纤维状星形胶质细胞肥大。从出生之日起进行原代培养的颤抖小鼠小脑在光学显微镜下显示髓鞘形成非常差。浦肯野细胞的轴突被认为是小脑原代培养物中主要的有髓轴突。来自颤抖者的浦肯野细胞在 Bodian Ag 浸渍、苏木精和伊红染色以及浦肯野细胞的 P400 蛋白表征方面表现正常。将颤抖者的条件培养基添加到对照培养物中不会干扰髓鞘形成。颤抖者中枢神经系统的髓鞘形成障碍可能是由少突胶质细胞的内在缺陷引起的，而不是由星形胶质细胞肥大或扩散因子引起的。
Dysmyelination in the CNS of shiverer mutant mice was studied in vivo and in vitro. By immunohistochemical reaction with glial fibrillary acidic protein antibody, hypertrophy of the fibrous astrocytes was observed in the white matter of shiverer cerebella. The cerebella of shiverer mice in primary culture from the day of birth showed very poor myelination under optical microscopy. Axons of Purkinje cells are thought to be the main myelinated axons in the primary culture of the cerebellum. Purkinje cells from shiverer appeared normal with regard to Bodian Ag impregnation, hematoxylin and eosin staining and P400 protein characterization of Purkinje cells. Addition of the conditioned culture medium of shiverer to the control culture did not interfere with myelination. The dysmyelination in the CNS of shiverer could be caused by an intrinsic defect of the oligodendrocyte rather than by hypertrophy of the astrocytes or by diffusible factors.