FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy
FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy
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DOI：
10.4161/cc.6344
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发表时间：
2008-08-01
期刊：
影响因子：
4.3
通讯作者：
De Laurenzi, Vincenzo
中科院分区：
文献类型：
--
作者：
Bongiorno-Borbone, Lucilla;De Cola, Antonella;De Laurenzi, Vincenzo
Cajal小体是真核细胞细胞核中包含的许多特殊细胞器之一，并参与许多功能，包括调节复制依赖性组蛋白基因转录。在正常的二倍体细胞中，它们的数量根据细胞周期的不同在0到4之间变化，尽管在癌细胞系中它们的数量变化很大，有人认为它与细胞倍性有关。在哺乳动物细胞中，就像在果蝇中一样，存在两个不同但功能相关的体：组蛋白基因位点体和Cajal体。前者可使用FLASH或NPAT作为标记进行检测，而后者则使用抗Coilin抗体进行标记。只有FLASH/NPAT组蛋白基因座体的数量与倍性相关，只有这些细胞器在细胞周期中受到调节。最后，我们发现这两个细胞器在细胞周期的S期完全共定位。
Cajal Bodies are one of many specialised organelles contained in the eukaryotic cell nucleus, and are involved in a number of functions, including regulation of replication-dependent histone gene transcription. In normal diploid cells their number varies between 0 and 4 depending on the cell cycle phase, although in cancer cell lines their number is extremely variable and it has been suggested that it correlates with cell ploidy.Here we show that in mammalian cells, as in Drosophila, two distinct though functionally related bodies exist: a histone gene locus body and a Cajal Body. The first one can be detected using FLASH or NPAT as markers while the second is labelled using antibodies against Coilin.Only the number of FLASH/NPAT histone gene locus bodies correlates with ploidy and only these organelles appear to be regulated during the cell cycle. Finally, we show that the two organelles completely co-localize during the S phase of the cell cycle.