Topical imiquimod yields systemic effects due to unintended oral uptake.
Topical imiquimod yields systemic effects due to unintended oral uptake.
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局部咪喹莫德（Imiquimod）由于意外口服吸收而产生全身效应。
DOI：
10.1038/srep20134
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发表时间：
2016-01-28
影响因子：
4.6
通讯作者：
Libert C
中科院分区：
文献类型：
--
作者：
Grine L;Steeland S;Van Ryckeghem S;Ballegeer M;Lienenklaus S;Weiss S;Sanders NN;Vandenbroucke RE;Libert C
重复应用局部咪喹莫特用作诱导小鼠银屑病样皮肤病变的实验模型。该模型的特征在于几种炎症过程，包括局部和全身的细胞因子产生、细胞浸润和脾肿大。为了研究I型干扰素响应于含有咪喹莫特的Aldara乳膏的产生，对IFNβ-荧光素酶报告小鼠进行体内和离体成像。发现I型干扰素在皮肤中产生，但也在小鼠无意中摄入咪喹莫特引起的肠道系统中产生。通过使用聚乙烯项圈防止摄入，这些影响，包括银屑病样病变几乎完全预防。我们的研究结果表明，局部使用Aldara治疗可诱导银屑病样皮肤炎症，但其作用方式取决于摄入化学物质，从而导致全身反应并影响局部炎症。因此，在皮肤疾病模型中评估皮肤炎症参数时，应考虑实验过程中局部治疗的潜在摄入。
Repetitive application of topical imiquimod is used as an experimental model for the induction of psoriasiform skin lesions in mice. The model is characterized by several inflammatory processes, including cytokine production both locally and systemically, cellular infiltration, and splenomegaly. To investigate the production of type I interferons in response to imiquimod-containing Aldara cream, IFNβ-luciferase reporter mice were imaged in vivo and ex vivo. Type I interferons were found to be produced in the skin, but also in the intestinal system caused by unintended ingestion of imiquimod by the mice. Through the use of Elizabethan collars to prevent ingestion, these effects, including psoriasiform lesions were nearly completely prevented. Our findings reveal that topical treatment with Aldara induces a psoriasiform skin inflammation, but that its mode of action depends on ingestion of the chemical, which leads to systemic responses and affects local inflammation. Therefore, potential ingestion of topical treatments during experimental procedures should be taken into account during assessment of cutaneous inflammatory parameters in skin disease models.