Inhibition of gastric mucin synthesis by Helicobacter pylori
Inhibition of gastric mucin synthesis by Helicobacter pylori
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DOI：
10.1016/s0016-5085(00)70360-x
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发表时间：
2000-06-01
期刊：
影响因子：
29.4
通讯作者：
Bresalier, RS
中科院分区：
文献类型：
--
作者：
Byrd, JC;Yunker, CK;Bresalier, RS
背景与目的：粘蛋白是一种高分子量的糖蛋白，具有保护胃上皮的作用。以往的资料表明，幽门螺杆菌感染患者胃表面型粘蛋白减少，并在根除感染后恢复。我们的目的是确定H.幽门螺杆菌对培养胃上皮细胞粘蛋白合成的影响研究方法：用[H-3]氨基葡萄糖标记和分子排阻层析法检测粘蛋白的合成，用Western blot法检测粘蛋白抗原MUC 5AC和MUC 1的表达。pylori，对粘蛋白分泌或降解没有影响，抑制作用迅速（4小时），部分可逆，取决于细菌浓度，并与不溶性膜部分相关。H.结论：幽门螺杆菌可抑制胃粘膜总粘蛋白的合成，并降低MUC 5AC和MUC 1的表达，胃粘膜粘蛋白合成的减少可能破坏粘膜保护层。
Background & Aims: Mucins are high-molecular-weight glycoproteins that protect the gastric epithelium. Previous data suggested that gastric surface-type mucin is decreased in Helicobacter pylori-infected patients and restored after eradication of the infection. Our aim was to determine the effect of H. pylori on mucin synthesis in cultured gastric epithelial cells. Methods: Mucin synthesis was measured by labeling with [H-3]glucosamine and size-exclusion chromatography, Expression of MUC5AC and MUC1 mucin protein antigens was quantitated by Western blot analysis, Results: Mucin synthesis was inhibited more than 80% when KATO III cells were incubated with H. pylori, with no effect on mucin secretion or degradation, Inhibition was rapid (4 hours), partially reversible, dependent on concentration of bacteria, and associated with the insoluble membrane fraction. H. pylori decreased levels of MUC5AC and MUC1 mucins, MUC1 inhibition was half-maximal by 4 hours and partially reversed by 24 hours, but the decrease in MUC5AC was less rapid and not reversible within 24 hours, Conclusions: H, pylori inhibits total mucin synthesis in vitro and decreases the expression of MUC5AC and MUC1, A decrease in gastric mucin synthesis in vivo may disrupt the protective surface mucin layer.