Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders
Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders
复制标题
DOI：
10.1007/978-3-319-52479-5_9
复制
发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Rahmoune, Hassan
中科院分区：
文献类型：
--
作者：
Guest, Paul C.;Rahmoune, Hassan
精神疾病的蛋白质组学生物标志物发现和验证的一个主要挑战是这些疾病背后固有的生物学复杂性。还有许多技术问题阻碍了这一过程，例如在临床前和临床环境中生物样品的取样、处理和储存缺乏标准化。本章描述了一种可重复的血清和血浆采样程序，该程序专为最大化二维凝胶电泳，多重免疫测定和质谱分析研究中的数据质量输出而设计。
A major challenge in proteomic biomarker discovery and validation for psychiatric diseases is the inherent biological complexity underlying these conditions. There are also many technical issues which hinder this process such as the lack of standardization in sampling, processing and storage of bio-samples in preclinical and clinical settings. This chapter describes a reproducible procedure for sampling blood serum and plasma that is specifically designed for maximizing data quality output in two-dimensional gel electrophoresis, multiplex immunoassay and mass spectrometry profiling studies.