IDENTIFICATION OF A MAJOR GROWTH-FACTOR FOR AIDS KAPOSIS-SARCOMA CELLS AS ONCOSTATIN-M
IDENTIFICATION OF A MAJOR GROWTH-FACTOR FOR AIDS KAPOSIS-SARCOMA CELLS AS ONCOSTATIN-M
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DOI：
10.1126/science.1542792
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发表时间：
1992-03-13
期刊：
影响因子：
56.9
通讯作者：
SARNGADHARAN, MG
中科院分区：
文献类型：
--
作者：
NAIR, BC;DEVICO, AL;SARNGADHARAN, MG
来自人类 T 细胞白血病病毒 2 型 (HTLV-II) 感染的 T 细胞的条件培养基支持源自获得性免疫缺陷综合征 (AIDS) 相关卡波西肉瘤病变的细胞（AIDS-KS 细胞）的生长和长期培养。从支持 AIDS-KS 细胞生长的条件培养基中纯化出 30 千道尔顿的蛋白质。该蛋白的氨基末端序列与制瘤素 M 的氨基末端序列相同，制瘤素 M 是一种抑制多种癌细胞生长的糖蛋白。当每毫升培养基中制瘤素 M 的浓度低于 1 纳克时，来自条件培养基的制瘤素 M 可刺激 AIDS-KS 细胞的生长增加两倍。
Conditioned medium from human T cell leukemia virus type 2 (HTLV-II)-infected T cells supports the growth and long-term culture of cells derived from acquired immunodeficiency syndrome (AIDS)-associated Kaposi's sarcoma lesions (AIDS-KS cells). A protein of 30 kilodaltons was purified from conditioned medium that supports the growth of AIDS-KS cells. The amino-terminal sequence of this protein was identical to the amino-terminal sequence of Oncostatin M, a glycoprotein that inhibits the growth of a variety of cancer cells. Oncostatin M from conditioned medium stimulated a twofold increase in the growth of AIDS-KS cells at a concentration of less than 1 nanogram of the protein per milliliter of medium.