Synapse loss may be a minor contributor to decreased regional cerebral blood flow in Alzheimer disease
Synapse loss may be a minor contributor to decreased regional cerebral blood flow in Alzheimer disease
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DOI：
10.1159/000067970
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Bonte, FJ
中科院分区：
文献类型：
--
作者：
Bigio, EH;Reisch, JS;Bonte, FJ
在这项研究中，我们测试的假设，突触损失有助于减少局部脑血流量（rCBF）在阿尔茨海默病（AD）。我们比较了13例AD患者的生前rCBF和死后突触素分析，作为突触丢失的一个指标。使用吸入氙气（Xe-133）进行rCBF研究，其产生定量结果。使用测量突触体素的酶联免疫吸附试验（ELISA）在死后脑样品中评价突触损失，结果表示为皮摩尔突触体素/10 mg脑。突触体素以浓度（QS法）或以浓度与额、颞、顶叶联合结果的比值（RS法）表示。使用QS方法，突触丢失与rCBF之间不存在相关性，使用RS方法，右侧SPECT与右侧颞叶突触素之间仅具有边缘显著性。这项研究的结果表明，突触损失可能是一个较小的贡献者减少脑血流量在AD中观察到。版权所有（C）2003 S. Karger AG，巴塞尔。
In this study we tested the hypothesis that synapse loss contributes to decreased regional cerebral blood flow (rCBF) in Alzheimer disease (AD). We compared ante-mortem rCBF and postmortem analysis of synaptophysin, as a measure of synapse loss, in 13 cases of AD. rCBF studies were performed using inhaled xenon gas (Xe-133), which yields quantitative results. Synapse loss was evaluated in postmortem brain samples using an enzyme-linked immunosorbant assay (ELISA) that measures synaptophysin, with results expressed as picomoles synaptophysin/10 mg brain. Synaptophysin was expressed either as concentration (QS method) or as the ratio of the concentration to the combined results in frontal, temporal and parietal lobe (RS method). There was no correlation between synapse loss and rCBF using the QS method and only borderline significance between right SPECT and right temporal synaptophysin using the RS method. The results of this study suggest that synapse loss may be a minor contributor to the decreased rCBF observed in AD. Copyright (C) 2003 S. Karger AG, Basel.