Transforming growth factors in the urine of normal, pregnant, and tumor-bearing humans.
Transforming growth factors in the urine of normal, pregnant, and tumor-bearing humans.
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正常人、孕妇和患有肿瘤的人尿液中的转化生长因子。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
G. Todaro
中科院分区：
文献类型：
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作者：
D. Twardzik;S. Sherwin;J. Ranchalis;G. Todaro
在正常、妊娠和荷瘤人的尿液中均可检测到转化生长因子（TGF）活性。这些酸稳定和热稳定的多肽竞争与表皮生长因子（EGF）膜受体结合，促进非转化啮齿动物细胞的非锚定生长。它们在表观分子量和软琼脂促生长活性方面与人EGF不同。妊娠女性尿液中含有TGF活性，表观分子量(s)（相对）（Mr）为10,000和17,000- 20,000。在肺癌患者的病例中，发现了大约30,000- 35,000 Mr的额外主要活动。所有检测的尿液标本还含有“常见的”8,000-Mr软琼脂促生长活性，该活性与EGF膜受体结合竞争，并且与EGF（尿胃泌素）在色谱上可分离。因此，尿液可能为这些tgf样活性的生化表征提供了方便和容易获得的来源，其中一些可能是某些类型癌症的临床有用的生物标志物。
Transforming growth factor (TGF) activities could be detected in the urine of normal, pregnant, and tumor-bearing humans. These acid- and heat-stable polypeptides competed for binding to epidermal growth factor (EGF) membrane receptors and promoted the anchorage-independent growth of nontransformed rodent cells. They differed from human EGF in their apparent molecular weights and soft-agar growth-stimulating activity. The urine from pregnant females contained TGF activities with apparent molecular weight(s) (relative) (Mr) of 10,000 ad 17,000--20,000. In the case of a lung cancer patient, an additional major activity of approximately 30,000--35,000 Mr was found. All urine specimens examined also contained a "common" 8,000-Mr soft-agar growth-stimulating activity, which competed for binding to EGF membrane receptors and which was chromatographically separable from EGF (urogastrone). Thus urine may provide a convenient and readily available source for the biochemical characterization of these TGF-like activities, some of which may be clinically useful biologic markers for certain types of cancer.