A graphene-based platform for induced pluripotent stem cells culture and differentiation
A graphene-based platform for induced pluripotent stem cells culture and differentiation
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DOI：
10.1016/j.biomaterials.2011.09.071
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发表时间：
2012-01-01
期刊：
影响因子：
14
通讯作者：
Hu, Y. -C.
中科院分区：
文献类型：
--
作者：
Chen, G. -Y.;Pang, D. W. -P.;Hu, Y. -C.
诱导多能干细胞（iPSC）作为再生医学的细胞来源具有很大的前景，但其培养，多能性的维持和分化的诱导仍然具有挑战性。相反，石墨烯（G）和氧化石墨烯（GO）在材料科学、物理、化学和纳米技术领域引起了极大的兴趣。在这里，我们报告了G和GO可以支持小鼠iPSCs培养并允许自发分化。有趣的是，G和GO表面导致不同的细胞增殖和分化特征。与玻璃表面相比，在G表面上培养的iPSC表现出相似程度的细胞粘附和增殖，而在GO表面上的iPSC以更快的速率粘附和增殖。此外，G有利地维持iPSC处于未分化状态，而GO加速分化。在G和GO表面上培养的iPSC自发分化为外胚层和中胚层谱系，没有显著差异，但G抑制iPSC向内胚层谱系分化，而GO增强内胚层分化。这些数据共同表明，G和GO的不同表面性质决定了iPSC的行为，并暗示了石墨烯基材料作为iPSC培养和多种应用平台的潜力。(C)2011爱思唯尔有限公司保留所有权利。
Induced pluripotent stem cells (iPSCs) hold great promise as a cell source for regenerative medicine yet its culture, maintenance of pluripotency and induction of differentiation remain challenging. Conversely, graphene (G) and graphene oxide (GO) have captured tremendous interests in the fields of materials science, physics, chemistry and nanotechnology. Here we report on that G and GO can support the mouse iPSCs culture and allow for spontaneous differentiation. Intriguingly, G and GO surfaces led to distinct cell proliferation and differentiation characteristics. In comparison with the glass surface, iPSCs cultured on the G surface exhibited similar degrees of cell adhesion and proliferation while iPSCs on the GO surface adhered and proliferated at a faster rate. Moreover, G favorably maintained the iPSCs in the undifferentiated state while GO expedited the differentiation. The iPSCs cultured on both G and GO surfaces spontaneously differentiated into ectodermal and mesodermal lineages without significant disparity, but G suppressed the iPSCs differentiation towards the endodermal lineage whereas GO augmented the endodermal differentiation. These data collectively demonstrated that the different surface properties of G and GO governed the iPSCs behavior and implicate the potentials of graphene-based materials as a platform for iPSCs culture and diverse applications. (C) 2011 Elsevier Ltd. All rights reserved.