Oscillating Cellulase Adsorption and Enhanced Lignocellulose Hydrolysis upon Ultrasound Treatment
Oscillating Cellulase Adsorption and Enhanced Lignocellulose Hydrolysis upon Ultrasound Treatment
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DOI：
10.1007/s12209-016-0019-9
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发表时间：
2017
影响因子：
7.1
通讯作者：
R. Su;Ren-bin Yang;Yan Jifeng;Ruoyu Du;R. Huang;W. Qi;Zhimin He
中科院分区：
文献类型：
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作者：
R. Su;Ren-bin Yang;Yan Jifeng;Ruoyu Du;R. Huang;W. Qi;Zhimin He
研究了超声波处理对纤维素酶吸附和木质纤维素水解的影响。低功率超声波（<120 W）处理后纤维素酶活性保持不变，而高功率超声波（>280 W）处理后纤维素酶活性下降。振荡纤维素酶吸附发生后，超声处理与任何强度。在50 °C、90 W超声处理1 h时，脱附和吸附的最大值分别为41.9%和83.1%。短时间和长时间超声实验之间的比较表明，超声处理倾向于从底物中解吸纤维素酶。然而，超声处理也导致生物质的进一步表面侵蚀，这增加了纤维素酶的可及性。超声处理的这些联合作用导致纤维素酶的振荡吸附。超声波处理引起的纤维素酶可及性的增加导致木质纤维素水解的显着增强。
We investigated the effects of ultrasound treatment on cellulase adsorption and lignocellulose hydrolysis. The activity of cellulase remained constant upon low-power ultrasound treatment (<120 W) and decreased using high-power ultrasound (>280 W). Oscillating cellulase adsorption occurred upon ultrasound treatment with any intensity. The maxima for desorption and adsorption were 41.9 and 83.1%, respectively, during 1 h of 90 W ultrasound treatment at 50 °C. A comparison between the short-time with long-time ultrasound experiments indicated that ultrasound treatment tended to desorb cellulase from substrate. However, ultrasound treatment also led to further surface erosion of biomass, which increased cellulase accessibility. These joint actions of ultrasound treatment induced the oscillating adsorption of cellulase. The increase in cellulase accessibility caused by ultrasound treatment led to a significant enhancement in lignocellulose hydrolysis.