Tissue engineering 2.0: guiding self-organization during pluripotent stem cell differentiation.
Tissue engineering 2.0: guiding self-organization during pluripotent stem cell differentiation.
复制标题
组织工程2.0：指导多能干细胞分化过程中的自组织。
DOI：
10.1016/j.copbio.2012.03.003
复制
发表时间：
2012
影响因子：
7.7
通讯作者：
Zandstra,PeterW
中科院分区：
文献类型：
--
作者：
Woodford,Curtis;Zandstra,PeterW
人多能干细胞(HPSC)的分化旨在利用生长因子或小分子以时间和剂量依赖的方式模拟发育。然而，使用这种方法产生的细胞类型在表型和功能上主要是胎儿样细胞，限制了它们在再生医学中的使用。在目前生产胰腺β细胞的努力中尤其如此，只有在移植到小鼠体内后，才能实现强大的胰腺祖细胞成熟。最近的研究表明，hPSC来源的细胞能够在体外自组织，这揭示了一种创造成熟细胞和组织的新范式。为发育中的幼稚细胞提供微妙和动态信号的组织工程策略可能被应用于在体外模拟胰腺发育的自组织方面。
Human pluripotent stem cell (hPSC) differentiation aims to mimic development using growth factors or small molecules in a time-dependent and dose-dependent manner. However, the cell types produced using this approach are predominantly fetal-like in phenotype and function, limiting their use in regenerative medicine. This is particularly true in current efforts to produce pancreatic beta cells, wherein robust pancreatic progenitor maturation can only be accomplished upon transplantation into mice. Recent studies have suggested that hPSC-derived cells are capable of self-organizing in vitro, revealing a new paradigm for creating mature cells and tissues. Tissue engineering strategies that provide subtle and dynamic signals to developmentally naïve cells may be applied to mimic in vitro the self-organization aspects of pancreatic development.