Function and transcriptome analysis of an oligopeptide transporter CgOPT2 in the rubber anthracnose fungus Colletotrichum gloeosporioides
Function and transcriptome analysis of an oligopeptide transporter CgOPT2 in the rubber anthracnose fungus Colletotrichum gloeosporioides
复制标题
橡胶炭疽菌胶孢炭疽菌寡肽转运蛋白 CgOPT2 的功能和转录组分析
DOI：
10.1016/j.pmpp.2021.101661
复制
发表时间：
2021-05-18
影响因子：
2.7
通讯作者：
Liu, Zhiqiang
中科院分区：
文献类型：
--
作者：
Li, Xiaoyu;Liu, Shayu;Liu, Zhiqiang
胶孢炭疽菌(Colletotrichum gloeosporioides)是引起橡胶炭疽病的主要病原菌，给天然橡胶工业带来巨大的经济损失。寡肽转运蛋白（OPT）是一种肽转运蛋白，通常在真菌中表现出多种生物学功能。在这项研究中，从橡胶炭疽真菌 C. gloeosporioides 中鉴定出寡肽转运蛋白基因 CgOPT2，它是酿酒酵母中 OPT2 的同源物。 CgOPT2 编码 990 个氨基酸的蛋白质，其 OPT 结构域包含 15 个跨膜结构域。 CgOPT2基因敲除突变体在PDA和CM培养基上的营养生长减少，并且比野生型菌株对H2O2、SDS和刚果红更敏感。此外，该突变体的分生孢子萌发明显滞后，对橡胶叶的毒力减弱。转录组分析显示CgOPT2可影响细胞转运、氨基酸代谢和信号转导等多个基因的表达。综上所述，CgOPT2是胶孢梭菌重要的寡肽转运蛋白，参与其营养生长、细胞壁完整性、氧化应激、分生孢子萌发和致病性等过程。
Colletotrichum gloeosporioides is a main pathogen causing rubber anthracnose, which brings huge economic loss to natural rubber industry. Oligopeptide transporter (OPT) is a kind of peptide transport protein, which usually exhibits various biological functions in fungi. In this study, an oligopeptide transporter gene CgOPT2 is identified from the rubber anthracnose fungus C. gloeosporioides as a homolog of OPT2 in Saccharomyces cerevisiae. CgOPT2 encodes a 990-amino acid protein, which has an OPT domain harboring 15 transmembrane domains. The gene knock-out mutant of CgOPT2 had reduced vegetative growth on PDA and CM media, and was more sensitive to H2O2, SDS and Congo Red than the wild-type strain. Moreover, the mutant had obviously lagged conidia germination and attenuated virulence on rubber leaves. Transcriptome analysis showed CgOPT2 could affect the expression of many genes involved in cellular transport, amino acid metabolism and signal transduction, etc. In conclusion, CgOPT2 is an important oligopeptide transporter of C. gloeosporioides, which participates in the process of vegetative growth, cell wall integrity, oxidative stress, conidia germination and pathogenicity.