Asynchronous glutamate release is enhanced in low release efficacy synapses and dispersed across the active zone.
Asynchronous glutamate release is enhanced in low release efficacy synapses and dispersed across the active zone.
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非同步谷氨酸释放在低释放效率突触中增强，并分散在整个活动区。
DOI：
10.1038/s41467-022-31070-4
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发表时间：
2022-06-17
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
神经递质的快速同步和延迟异步释放之间的平衡在定义神经元突触的计算性质和神经元网络活动的调节中具有重要作用。然而，它在单个突触水平上是如何调节的仍然知之甚少。在这里，使用荧光谷氨酸传感器SF-iGluSnFR，我们以4毫秒的时间和75 nm的空间分辨率对单个锥体细胞的数十至数百个单个突触输出中的量子囊泡释放进行成像。我们发现，同步和异步突触囊泡胞吐之间的比例变化很大，由同一轴突供应的突触之间，并在低释放概率突触的同步性释放减少。我们进一步证明，异步胞吐网站更广泛地分布在释放区域内比同步网站。总之，我们的研究结果揭示了突触的两个主要功能特性-神经递质释放的时间和整体功效之间的普遍关系。神经递质的释放可以相对于动作电位延迟。这项工作演示了这种异步释放是如何与整体囊泡释放概率和短期可塑性。
The balance between fast synchronous and delayed asynchronous release of neurotransmitters has a major role in defining computational properties of neuronal synapses and regulation of neuronal network activity. However, how it is tuned at the single synapse level remains poorly understood. Here, using the fluorescent glutamate sensor SF-iGluSnFR, we image quantal vesicular release in tens to hundreds of individual synaptic outputs from single pyramidal cells with 4 millisecond temporal and 75 nm spatial resolution. We find that the ratio between synchronous and asynchronous synaptic vesicle exocytosis varies extensively among synapses supplied by the same axon, and that the synchronicity of release is reduced at low release probability synapses. We further demonstrate that asynchronous exocytosis sites are more widely distributed within the release area than synchronous sites. Together, our results reveal a universal relationship between the two major functional properties of synapses – the timing and the overall efficacy of neurotransmitter release. Neurotransmitters can be released with a delay in relation to action potentials. This work demonstrates how this asynchronous release is related to overall vesicle release probability and short-term plasticity.
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DOI：
10.1002/syn.22178
发表时间：
2020-12
期刊：
Synapse (New York, N.Y.)
影响因子：
--
作者：
Chamberland S;Timofeeva Y;Evstratova A;Norman CA;Volynski K;Tóth K
通讯作者：
Tóth K
影响因子：
--
作者：
Cowan, Ann E.;Moraru, Ion I.;Schaff, James C.;Slepchenko, Boris M.;Loew, Leslie M.
通讯作者：
Loew, Leslie M.
影响因子：
64.5
作者：
GEPPERT, M;GODA, Y;SUDHOF, TC
通讯作者：
SUDHOF, TC
影响因子：
4.8
作者：
Bansaghi, Szava;Golenar, Tuende;Hajnoczky, Gyoergy
通讯作者：
Hajnoczky, Gyoergy
影响因子：
5.3
作者：
Eltes, Timea;Kirizs, Tekla;Holderith, Noemi
通讯作者：
Holderith, Noemi