Cloned measles virus-specific T lymphocytes from a twin with multiple sclerosis.
Cloned measles virus-specific T lymphocytes from a twin with multiple sclerosis.
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来自患有多发性硬化症的双胞胎的克隆麻疹病毒特异性 T 淋巴细胞。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
P. Lake
中科院分区：
文献类型：
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作者：
J. Richert;H. McFarland;D. McFarlin;W. Bellini;P. Lake
一个麻疹病毒特异性T淋巴细胞克隆来自麻疹反应与多发性硬化症的双胞胎已经产生。克隆的细胞对麻疹病毒有反应，但对腮腺炎、牛痘或犬瘟热病毒没有反应。克隆识别分布在麻疹病毒感染细胞的膜和细胞质组分中的抗原。从自体受试者和她的健康、麻疹无应答者、HLA同卵双胞胎中获得的经辐照的单核细胞同样良好地将抗原呈递给克隆。对一组HLA型辐射单核细胞的研究表明，抗原是在与HLA-DRw 2相连但不相同的基因产物的背景下呈递给克隆的。该克隆产生白细胞介素2，并具有被OKT-3、OKT-4和抗HLA-DR单克隆抗体识别的表面决定簇，但不被OKT-8识别。因此，它似乎属于T淋巴细胞的辅助/诱导亚群。
A measles virus-specific T lymphocyte clone derived from a measles-responder twin with multiple sclerosis has been produced. The cloned cells proliferate in response to measles virus but not mumps, vaccinia, or canine distemper viruses. The clone recognizes an antigen that is distributed in both the membrane and cytoplasmic fractions of cells infected with measles virus. Antigen is presented to the clone equally well by irradiated mononuclear cells obtained from the autologous subject and from her healthy, measles-nonresponder, HLA-identical twin. Studies with a panel of HLA-typed irradiated mononuclear cells indicate that antigen is presented to the clone in the context of a gene product linked to, but not identical with, HLA-DRw2. The clone produces interleukin 2 and has surface determinants recognized by OKT-3, OKT-4, and anti-HLA-DR monoclonal antibodies, but not by OKT-8. It thus appears to belong to the helper/inducer subpopulation of T lymphocytes.