Campylobacter jejuni 11168H Exposed to Penicillin Forms Persister Cells and Cells With Altered Redox Protein Activity.
Campylobacter jejuni 11168H Exposed to Penicillin Forms Persister Cells and Cells With Altered Redox Protein Activity.
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DOI：
10.3389/fcimb.2020.565975
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发表时间：
2020
影响因子：
5.7
通讯作者：
Titball RW
中科院分区：
文献类型：
--
作者：
Morcrette H;Kovacs-Simon A;Tennant RK;Love J;Wagley S;Yang ZR;Studholme DJ;Soyer OS;Champion OL;Butler CS;Titball RW
持久性细胞的形成是细菌在环境压力下存活的一种机制。我们发现，在暴露于100 × MIC的青霉素G 24小时后，空肠弯曲杆菌11168H以10−3的频率形成持久细胞。用氧化还原敏感的荧光染料染色细胞群显示，青霉素G处理导致细胞群的荧光增强。我们提出的证据表明，这可能是电子传递链中氧化还原蛋白活性增加或与之相关的结果。这些数据表明，青霉素G处理的空肠梭菌细胞群体可能经历电子传递链的重塑，以调节膜超极化和细胞内碱化；因此降低了抗生素的功效，并可能有助于持久细胞的形成。
The formation of persister cells is one mechanism by which bacteria can survive exposure to environmental stresses. We show that Campylobacter jejuni 11168H forms persister cells at a frequency of 10−3 after exposure to 100 × MIC of penicillin G for 24 h. Staining the cell population with a redox sensitive fluorescent dye revealed that penicillin G treatment resulted in the appearance of a population of cells with increased fluorescence. We present evidence, to show this could be a consequence of increased redox protein activity in, or associated with, the electron transport chain. These data suggest that a population of penicillin G treated C. jejuni cells could undergo a remodeling of the electron transport chain in order to moderate membrane hyperpolarization and intracellular alkalization; thus reducing the antibiotic efficacy and potentially assisting in persister cell formation.