HORIZONTAL COMPARTMENTATION IN THE GERMINAL MATRICES AND INTERMEDIATE ZONE OF THE EMBRYONIC RAT CEREBRAL-CORTEX
HORIZONTAL COMPARTMENTATION IN THE GERMINAL MATRICES AND INTERMEDIATE ZONE OF THE EMBRYONIC RAT CEREBRAL-CORTEX
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DOI：
10.1016/0014-4886(90)90061-v
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发表时间：
1990-01-01
影响因子：
5.3
通讯作者：
BAYER, SA
中科院分区：
文献类型：
--
作者：
ALTMAN, J;BAYER, SA
在注射[3 H]胸苷后存活1天或莫尔时间的大鼠胚胎的胚基质和大脑皮质中间区中研究了细胞区室化。与短期生存胸苷放射自显影在神经上皮中观察到的垂直间隔形成鲜明对比，连续生存放射自显影显示生发基质和中间区均存在水平间隔。在存活24小时的胚胎的神经上皮中，差异标记的细胞形成两个不同的水平带。与有丝分裂区重叠的带由重标记细胞组成，而与合成区重叠的带由轻标记细胞组成。这表明在神经上皮内有两个增殖细胞群，一个快速翻转，另一个更慢。在同一胚胎的皮层中间区，存在几条水平带。其中，两个带的高度标记的细胞的处置-上级带和下级带-被跟踪了几天。上级带显然是由神经胶质细胞组成的，这些神经胶质细胞向内囊和胼胝体的方向分散。相反，下带（与室管膜下区重叠，在室管膜下区有许多细胞水平取向）显然由寄居的年轻神经元组成。下带的细胞在暂停1-2天后恢复向皮质板的迁移。这些观察结果要求重新评估年轻皮层神经元沿着直接径向路径到达皮层板的观点。
Cellular compartmentation was studied in the germinal matrices and the intermediate zone of the cerebral cortex of rat embryos that survived for 1 or mor days after injection with [3H]thymidine. In contrast to the vertical compartmentation seen in the neuroepithelium with short-survival thymidine autoradiography, sequential-survival autoradiography revealed a horizontal compartmentation both in the germinal matrices and the intermediate zone. In the neuroepithelium of embryos that survived for 24 h, the differentially labeled cells form two distinct horizontal bands. The band overlapping with the mitotic zone is composed of heavily labeled cells, whereas the band overlapping with the synthetic zone is composed of lightly labeled cells. This indicates that there are two proliferative cell populations within the neuroepithelium, one turning over fast and the other more slowly. In the cortical intermediate zone of the same embryos several horizontal bands are present. Of these, the dispositions of two bands of heavily labeled cells-the superior band and the inferior band-were followed for several days. The superior band is apparently composed of glial cells that disperse in the direction of the internal capsule and the corpus callosum. In contrast, the inferior band (which overlaps with the subventricular zone where many cells are horizontally oriented) is apparently composed of sojourning young neurons. The cells of the inferior band resume their migration toward the cortical plate after a pause of 1-2 days. These observations call for a reappraisal of the view that young cortical neurons follow a direct radial path to the cortical plate.