Food with calorie restriction reduces the development of atherosclerosis in apoE-deficient mice
Food with calorie restriction reduces the development of atherosclerosis in apoE-deficient mice
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限制热量的食物可减少 apoE 缺陷小鼠动脉粥样硬化的发展
DOI：
10.1016/j.bbrc.2020.01.109
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发表时间：
2020
影响因子：
3.1
通讯作者：
Chen Yuanli
中科院分区：
文献类型：
--
作者：
Yang Jie;Zeng Peng;Liu Lipei;Yu Miao;Su Jiamin;Yan Yali;Ma Jialing;Hu Wenquan;Yang Xiaoxiao;Han Jihong;Duan Yajun;Chen Yuanli
热量限制（CR）可以改善包括心血管疾病在内的各种疾病。然而，其对动脉粥样硬化的保护作用及其机制尚未完全阐明。在这项研究中，我们在对照组中喂养apoE缺陷（apoE−/-）小鼠，高脂肪饮食（HFD，21%脂肪加0.5%胆固醇）或CR组中喂养CR饮食（CRD，2%脂肪加0.5%胆固醇，≤ 40%卡路里限制和与HFD相同的胆固醇，维生素，矿物质和氨基酸水平）。喂养16周后，与HFD相比，CRD显著降低了小鼠的动脉粥样硬化。CRD增加SMC和胶原含量，但减少巨噬细胞含量，坏死核心和血管钙化。从机制上讲，CRD减弱体重增加，改善脂质谱，但对巨噬细胞脂质代谢几乎没有影响。CRD还抑制病变中炎性分子的表达。总之，我们的研究表明CRD有效地减少了apoE−/-小鼠的动脉粥样硬化，表明它是动脉粥样硬化管理的有效和可重复的治疗方法。
Calorie restriction (CR) ameliorates various diseases including cardiovascular disease. However, its protection and underlying mechanisms against atherosclerosis remain un-fully elucidated. In this study, we fed apoE deficient (apoE−/-) mice in Control group a high-fat diet (HFD, 21% fat plus 0.5% cholesterol) or in CR group a CR diet (CRD, 2% fat plus 0.5% cholesterol, ∼40% calorie restriction and same levels of cholesterol, vitamins, minerals and amino acids as in HFD). After 16 weeks feeding, compared with HFD, CRD substantially reduced atherosclerosis in mice. CRD increased SMC and collagen content but reduced macrophage content, necrotic core and vascular calcification in lesion areas. Mechanistically, CRD attenuated bodyweight gain, improved lipid profiles but had little effect on macrophage lipid metabolism. CRD also inhibited expression of inflammatory molecules in lesions. Taken together, our study demonstrates CRD effectively reduces atherosclerosis in apoE−/-mice, suggesting it as a potent and reproducible therapy for atherosclerosis management.