Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis
Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis
复制标题
生长素通过诱导拟南芥中 ARF 介导的 ABI3 激活来刺激脱落酸信号传导来控制种子休眠
DOI：
10.1073/pnas.1304651110
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发表时间：
2013-09-17
影响因子：
11.1
通讯作者：
He, Zu-Hua
中科院分区：
文献类型：
--
作者：
Liu, Xiaodong;Zhang, Hong;He, Zu-Hua
种子休眠是植物生命周期中的一个关键步骤，对植物物种的生存至关重要;这个过程对于农业实践也很重要，以防止收获前潮湿条件下的发芽。这项研究揭示了一个以前未被认识的行动，生长素在维持种子休眠，通过生长素反应因子10/16介导的表达脱落酸不敏感3，脱落酸介导的种子休眠的关键调节。种子从休眠到萌发的转变是植物生命周期中的一个重要生理过程。脱落酸（阿坝）是唯一的植物激素已知保持种子休眠;它通过涉及转录因子脱落酸不敏感3（ABI 3）的基因表达网络发挥作用。然而，是否其他植物激素途径的功能，在维持种子休眠，以响应环境和内部信号仍然是一个重要的问题。在这里，我们表明，植物生长激素生长素，作为一个多功能的触发器在许多发展过程中，也发挥了关键作用，在拟南芥种子休眠。我们发现，生长素信号在MIR 160过表达植物，生长素受体突变体，或生长素生物合成突变体显着释放种子休眠中断，而生长素信号或生物合成的增加大大提高种子休眠。生长素在种子休眠中的作用需要阿坝信号通路（反之亦然），表明生长素和阿坝在种子休眠中的作用是相互依赖的。此外，我们表明，生长素的行为上游的主要调节种子休眠，ABI 3，通过招募生长素反应因子生长素反应因子10和生长素反应因子16控制ABI 3在种子萌发过程中的表达。因此，我们的研究揭示了一个以前未被认识到的种子休眠调节因子和生长素和阿坝信号在这一重要过程中的协调网络。
Significance Seed dormancy is a critical step in the lifecycle of plants, and it is crucial to the survival of plant species; this process is also important for agricultural practice to prevent preharvest sprouting when humid conditions persist before harvest. This study uncovers a previously unrecognized action of auxin in maintaining seed dormancy through AUXIN RESPONSE FACTOR 10/16-mediated expression of ABSCISIC ACID INSENSITIVE 3, a key regulator in the abscisic acid-mediated seed dormancy. The transition from dormancy to germination in seeds is a key physiological process during the lifecycle of plants. Abscisic acid (ABA) is the sole plant hormone known to maintain seed dormancy; it acts through a gene expression network involving the transcription factor ABSCISIC ACID INSENSITIVE 3 (ABI3). However, whether other phytohormone pathways function in the maintenance of seed dormancy in response to environmental and internal signals remains an important question. Here, we show that the plant growth hormone auxin, which acts as a versatile trigger in many developmental processes, also plays a critical role in seed dormancy in Arabidopsis. We show that disruptions in auxin signaling in MIR160-overexpressing plants, auxin receptor mutants, or auxin biosynthesis mutants dramatically release seed dormancy, whereas increases in auxin signaling or biosynthesis greatly enhance seed dormancy. Auxin action in seed dormancy requires the ABA signaling pathway (and vice versa), indicating that the roles of auxin and ABA in seed dormancy are interdependent. Furthermore, we show that auxin acts upstream of the major regulator of seed dormancy, ABI3, by recruiting the auxin response factors AUXIN RESPONSE FACTOR 10 and AUXIN RESPONSE FACTOR 16 to control the expression of ABI3 during seed germination. Our study, thus, uncovers a previously unrecognized regulatory factor of seed dormancy and a coordinating network of auxin and ABA signaling in this important process.