Three-Dimensional Co-Cultures of Osteoblasts and Endothelial Cells in DegraPol Foam: Histological and High-Field Magnetic Resonance Imaging Analyses of Pre-Engineered Capillary Networks in Bone Grafts
Three-Dimensional Co-Cultures of Osteoblasts and Endothelial Cells in DegraPol Foam: Histological and High-Field Magnetic Resonance Imaging Analyses of Pre-Engineered Capillary Networks in Bone Grafts
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DOI：
10.1089/ten.tea.2010.0278
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发表时间：
2011-02-01
影响因子：
4.1
通讯作者：
Calcagni, Maurizio
中科院分区：
文献类型：
--
作者：
Buschmann, Johanna;Welti, Manfred;Calcagni, Maurizio
使用 DegraPol (R) 泡沫作为支架材料，在小鸡绒毛尿囊膜模型的临界尺寸模型中解决了骨移植的组织工程问题。支架分别接种了人成骨细胞和人内皮细胞的培养物，或两种细胞类型的共培养物（对照：无细胞）。通过高场磁共振成像 (MRI) 和组织学分析体外样品（培养 7 天）和孵化第 15 天的离体绒毛尿囊膜模型样品。使用钆二乙烯三胺五乙酸 (DTPA) 进行对比增强 MRI 评估，共培养系统在灌注方面表现最佳。组织学分析证实，通过共培养接种的支架支持血管向内生长的增加。 DegraPol 泡沫是骨组织工程的合适支架，MRI 技术可以在骨形成结构的早期阶段对灌注能力进行无损和定量评估。
Tissue engineering of bone grafts was addressed in a critical-sized model on the chick chorioallantoic membrane model, using DegraPol (R) foam as scaffold material. The scaffolds were seeded with cultures of human osteoblasts and human endothelial cells, respectively, or with a co-culture of the two cell types (control: no cells). In vitro samples (7 days cultivation) and ex vivo chorioallantoic membrane model samples at incubation day 15 were analyzed by high-field magnetic resonance imaging (MRI) and histology. The co-culture system performed best with respect to perfusion, as assessed by contrast-enhanced MRI using gadolinium-diethylene-triamine-pentaacetic acid (DTPA). The scaffold seeded by the co-culture supported an increased vascular ingrowth, which was confirmed by histological analysis. DegraPol foam is a suitable scaffold for bone tissue engineering and the MRI technique allows for nondestructive and quantitative assessment of perfusion capability during early stages of bone forming constructs.