Eotaxin-1,-2, and -3 Immunoreactivity and Protein Concentration in the Nasal Polyps of Eosinophilic Chronic Rhinosinusitis Patients
Eotaxin-1,-2, and -3 Immunoreactivity and Protein Concentration in the Nasal Polyps of Eosinophilic Chronic Rhinosinusitis Patients
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DOI：
10.1002/lary.20191
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发表时间：
2009-06-01
期刊：
影响因子：
2.6
通讯作者：
Ikeda, Katsuhisa
中科院分区：
文献类型：
--
作者：
Yao, Toru;Kojima, Yuko;Ikeda, Katsuhisa
目的/假设：嗜酸性粒细胞性慢性鼻窦炎（CRS）的特征是大量嗜酸性粒细胞在鼻窦粘膜和鼻息肉中聚集，往往很难控制，即使是手术。本研究的目的是评估嗜酸性粒细胞趋化因子的表达和定位，这是众所周知的是有效的和选择性的嗜酸性粒细胞在CRS.Study设计：Randomized study.Methods：患者被分为嗜酸性粒细胞和noneosinophilic组。苏木精-伊红染色观察鼻息肉的组织学形态。Eotaxin-1、Eotaxin-2、Eotaxin-3在鼻息肉组织中呈免疫组化染色。此外，使用酶联免疫吸附试验（ELISA）测定鼻息肉和鼻窦积液内的嗜酸性粒细胞活化趋化因子亚型的蛋白质含量。结论：目前的研究结果表明，增强嗜酸性粒细胞的嗜酸性粒细胞家族生产的结果在招聘嗜酸性粒细胞进入组织的自我扩增过程。
Objectives/Hypothesis: Eosinophilic chronic rhinosinusitis (CRS) is characterized by the accumulation of numerous eosinophils in the sinus mucosa and nasal polyps, which. are frequently difficult to control, even with surgery. The present study was designed to evaluate the expression and localization of eotaxins, which are well known to be potent and selective chemoattractants for eosinophils in CRS.Study Design: Randomized study.Methods: The patients were classified into eosinophilic and noneosinophilic groups. Histopathological profiles of the nasal polyp were observed with hematoxylin-eosin staining. Eotaxin-1, -2, and -3 were immunohistochemically stained in the nasal polyps. Furthermore, the protein content of eotaxin subtypes inside the nasal polyp and sinus effusion was measured using enzyme-linked immunosorbent assay (ELISA).Conclusions: The present findings suggest that enhanced eotaxin family production by eosinophils results in the recruitment of eosinophils into the tissue by a self-amplifying process.