FISSION YEAST GENES THAT CONFER RESISTANCE TO STAUROSPORINE ENCODE AN AP-1-LIKE TRANSCRIPTION FACTOR AND A PROTEIN-KINASE RELATED TO THE MAMMALIAN ERK1/MAP2 AND BUDDING YEAST FUS3 AND KSS1 KINASES
FISSION YEAST GENES THAT CONFER RESISTANCE TO STAUROSPORINE ENCODE AN AP-1-LIKE TRANSCRIPTION FACTOR AND A PROTEIN-KINASE RELATED TO THE MAMMALIAN ERK1/MAP2 AND BUDDING YEAST FUS3 AND KSS1 KINASES
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DOI：
10.1101/gad.5.1.60
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发表时间：
1991-01-01
影响因子：
10.5
通讯作者：
YANAGIDA, M
中科院分区：
文献类型：
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作者：
TODA, T;SHIMANUKI, M;YANAGIDA, M
Staurosporine是一种有效的蛋白激酶C抑制剂，在细胞分裂后的一个阶段特异性地阻止分裂酵母细胞的伸长。我们分离了两个基因，当它们携带在多拷贝质粒上时，在裂变酵母中赋予耐药性。其中一个是spk1+，其编码的蛋白激酶与哺乳动物ERK1/MAP2激酶和出芽酵母KSS1和FUS3基因编码的蛋白激酶高度相似（同源性为54%）。它不是裂糖酵母pombe细胞营养生长所必需的，而是接合所必需的。spk1+基因产物是一种富集于细胞核的45-kD蛋白，加入staurosporine后其表达量增加10倍。另一个基因pap1+编码一个ap -1样转录因子，该转录因子包含一个富含碱性氨基酸的区域，随后是一个“亮氨酸拉链”基序。pap1+基因是spk1+赋予的耐药所必需的。这两个基因似乎是分裂酵母生长控制途径的一部分。
Staurosporine, a potent inhibitor of protein kinase C, arrests fission yeast cell elongation specifically at a stage immediately after cell division. We isolated two genes, which, when carried on multicopy plasmids, confer drug resistance in fission yeast. One, spk1+, encodes a protein kinase highly similar (54% identity) to those encoded by the mammalian ERK1/MAP2 kinase and the budding yeast KSS1 and FUS3 genes. It is not essential for vegetative growth of Schizosaccharomyces pombe cells but is required for conjugation. The spk1+ gene product is a 45-kD protein enriched in the nucleus, and its level increases 10-fold after addition of staurosporine. The other gene pap1+ encodes an AP-1-like transcription factor that contains a region rich in basic amino acids followed by a "leucine zipper" motif. The pap1+ gene is required for spk1+-conferred staurosporine resistance. These two genes appear to function as a part of the fission yeast growth control pathway.