DETECTION OF THE TUMOR-ASSOCIATED ANTIGENS RECOGNIZED BY THE MONOCLONAL-ANTIBODIES HMFG-1 AND HMFG-2 IN SERUM FROM PATIENTS WITH BREAST-CANCER
DETECTION OF THE TUMOR-ASSOCIATED ANTIGENS RECOGNIZED BY THE MONOCLONAL-ANTIBODIES HMFG-1 AND HMFG-2 IN SERUM FROM PATIENTS WITH BREAST-CANCER
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DOI：
10.1002/ijc.2910340605
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发表时间：
1984-01-01
影响因子：
6.4
通讯作者：
TAYLORPAPADIMITRIOU, J
中科院分区：
文献类型：
--
作者：
BURCHELL, J;WANG, D;TAYLORPAPADIMITRIOU, J
The levels of the human milk fat globule [HMFG] 1 and 2 antigens were measured in the sera of patients with advanced breast cancer, using a sandwich type radioimmune assay which exploits the carbohydrate nature of the antigenic determinants. In the series studied, 30% of sera from advanced breast cancer patients contained elevated levels of the HMFG-1 antigens as compared with 6% of sera from healthy control women; 53% of the advanced breast cancer patients showed elevated levels of HMFG-2 antigen compared with 16.6% of the controls. By means of the immune blotting technique, the components carrying the antigenic determinants in sera were identified and compared for size with those molecules expressing the determinants in primary and secondary breast tumors. Both antibodies react with similar MW components of 320,000 (320 kd) and 280 kd which are present in serum and tumor samples, although lower MW bands of 230 kd and 190 kd can be seen in some tumors. These components are much smaller than the glycoprotein (> 400 kd) present in the human milk fat globule which carries the antigenic determinants recognized by HMFG-1 and -2.
