Site-specific PEGylation of proteins containing unnatural amino acids
Site-specific PEGylation of proteins containing unnatural amino acids
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DOI：
10.1016/j.bmcl.2004.09.059
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发表时间：
2004-12-06
影响因子：
2.7
通讯作者：
Schultz, PG
中科院分区：
文献类型：
--
作者：
Deiters, A;Cropp, TA;Schultz, PG
在这里，我们报告了一个普遍适用的聚乙二醇化方法的基础上，对叠氮苯丙氨酸到酵母中的蛋白质的位点特异性掺入。叠氮基用于与炔衍生的PEG试剂的温和的[3+2]环加成反应，以提供选择性聚乙二醇化的蛋白质。这种策略对于产生用于治疗应用的选择性聚乙二醇化蛋白质应该是有用的。(C)2004爱思唯尔有限公司保留所有权利。
Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications. (C) 2004 Elsevier Ltd. All rights reserved.