Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation
Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation
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DOI：
10.1016/j.chroma.2004.12.014
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发表时间：
2005-02-04
影响因子：
4.1
通讯作者：
Horák, D
中科院分区：
文献类型：
--
作者：
Krízová, J;Spanová, A;Horák, D
含磁性和非磁性微球的羧基被用于基因组DNA的固相可逆固定化（SPR1）。在胶体氧化铁存在下，通过分散共聚制备了具有亲水性的磁性无孔聚（2-羟乙基甲基丙烯酸-共二甲基丙烯酸乙酯）- P（HEMA-co-EDMA）、聚（甲基丙烯酸缩水甘油酯）- PGMA和P（HEMA-co-GMA）微球。用鸡红细胞DNA和长双歧杆菌细胞DNA检测了磁性微球的吸附/解吸性能。利用SPRI解决了DNA样品中细胞外抑制剂引起的聚合酶链反应（PCR）假阴性结果的发生。采用P（HEMA-co-EDMA）微球和P（HEMA-co-GMA）微球分别从不同乳制品中分离双歧杆菌DNA，并进行PCR鉴定。(C) 2004 Elsevier B.V.版权所有
Carboxyl groups containing magnetic and non-magnetic microspheres were used in solid-phase reversible immobilization (SPR1) of genomic DNA. Magnetic non-porous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), poly(glycidyl methacrylate) - PGMA and P(HEMA-co-GMA) microspheres with hydrophilic properties were prepared by dispersion copolymerization of the respective monomers in the presence of colloidal iron oxides. DNA from chicken erythrocytes and DNA isolated from bacterial cells of Bifidobacterium longum was used for testing of adsorption/desorption properties of magnetic microspheres. The occurrence of false negative results in polymerase chain reaction (PCR) caused by the presence of extracellular inhibitors in DNA samples has been solved using SPRI. The P(HEMA-co-EDMA) and P(HEMA-co-GMA) microspheres were used for isolation of DNA from different dairy products followed by PCR identification of Bifidobacterium strains. (C) 2004 Elsevier B.V. All rights reserved.