Hzf protein regulates dendritic localization and BDNF-induced translation of type 1 inositol 1,4,5-trisphosphate receptor mRNA
Hzf protein regulates dendritic localization and BDNF-induced translation of type 1 inositol 1,4,5-trisphosphate receptor mRNA
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DOI：
10.1073/pnas.0504684102
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发表时间：
2005-11-22
影响因子：
11.1
通讯作者：
Okano, H
中科院分区：
文献类型：
--
作者：
Iijima, T;Imai, T;Okano, H
某些mRNA在树突的定位及其在突触区域的局部翻译被认为参与了突触可塑性的某些方面。靶向mRNA的3'非翻译区（3' UTR）内的顺式作用元件，其被反式作用RNA结合蛋白结合，控制树突状mRNA定位。在这里，我们确定了造血锌指（Hzf）作为一种反式作用因子，通过与3' UTR结合来调节1型肌醇1，4，5-三磷酸受体（IP 3RI）的树突状mRNA定位，IP 3RI是小脑浦肯野细胞中树突状定位的mRNA。在Hzf缺陷小鼠中，IP 3RI mRNA的树突定位和脑源性神经营养因子诱导的IP 3RI蛋白在小脑中的合成受损。这些结果表明，Hzf是一种RNA结合蛋白，控制树突状mRNA的定位和活性依赖性翻译的IP 3RI，并可能参与突触可塑性的某些方面。
The localization of certain mRNAs to dendrites and their local translation in synaptic regions are proposed to be involved in certain aspects of synaptic plasticity. A cis-acting element within the 3' untranslated region (3' UTR) of the targeted mRNAs, which is bound by a trans-acting RNA-binding protein, controls the dendritic mRNA localization. Here, we identified hematopoietic zinc finger (Hzf) as a trans-acting factor that regulates the dendritic mRNA localization of the type 1 inositol 1,4,5-trisphosphate receptor (IP3RI), a dendritically localized mRNA in cerebellar Purkinje cells, via binding to the 3' UTR. In Hzf-deficient mice, the dendritic localization Of IP3RI mRNA and brain-derived neurotrophic factor-induced IP3RI protein synthesis in the cerebellum were impaired. These findings suggest that Hzf is an RNA-binding protein that controls the dendritic mRNA localization and activity-dependent translation of IP3RI, and may be involved in some aspects of synaptic plasticity.