HUMAN PARA-INFLUENZA VIRUS TYPE-3 - MESSENGER-RNAS, POLYPEPTIDE CODING ASSIGNMENTS, INTERGENIC SEQUENCES, AND GENETIC-MAP
HUMAN PARA-INFLUENZA VIRUS TYPE-3 - MESSENGER-RNAS, POLYPEPTIDE CODING ASSIGNMENTS, INTERGENIC SEQUENCES, AND GENETIC-MAP
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DOI：
10.1128/jvi.59.3.646-654.1986
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发表时间：
1986-09-01
影响因子：
5.4
通讯作者：
COLLINS, PL
中科院分区：
文献类型：
--
作者：
SPRIGGS, MK;COLLINS, PL
通过体外翻译的杂交停滞和杂交选择，鉴定了人副流感病毒3型(PF3)主要核衣壳蛋白(NP)、核衣壳P蛋白+非结构C蛋白(P+C)和基质蛋白(M)的mRNAs克隆。以前，血凝素神经氨酸酶糖蛋白(HN)和融合糖蛋白(F)mRNAs(N.Elango，J.E.Coligan，R.C.Jambou，S.Venkatesan，J.Virol)的cDNA克隆已被鉴定和测序。57：481-489,1986；M.K.Spriggs，R.A.Olmsted，S.Venkatesan，J.E.Coligan，和P.L.Collins，病毒学152：241-251,1986)。根据cDNA的核苷酸序列设计合成的寡核苷酸，用于指导PF3基因组RNA(VRNA)中基因连接的双脱氧核苷酸测序。从vRNA测序中，通过人工合成的寡核苷酸与凝胶电泳法分离的胞内PF3mRNAs杂交，鉴定出第六个病毒基因为大核衣壳蛋白(L)基因。6个PF3基因在vRNA上的顺序为3‘’-NP-P+C-M-F-HN-L-5‘’。5个基因间隔区由3‘’-GAA三核苷酸组成。PF3基因以半服务的10个核苷酸的基因起始序列开始，以半服务的12个核苷酸的基因结束序列终止。M基因以异常的基因末端序列终止；对细胞内mRNA的分析表明，这种异常序列与通读mRNA的高积累不成比例地相关。这些研究表明，PF3编码6个独特的mRNAs(NP、P+C、M、F、HN和L)，编码7种蛋白(NP、P、C、M、F、HN和L)，为PF3与仙台(小鼠副流感1型)病毒的密切关系提供了证据。
cDNA clones of mRNAs for the major nucleocapsid protein (NP), the nucleocapsid P protein plus the nonstructural C protein (P+C), and the matrix protein (M) of human parainfluenza virus type 3 (PF3) were identified by hybrid arrest and hybrid selection of in vitro translation. Previously, cDNA clones were identified and sequenced for the hemagglutinin-neuraminidase glycoprotein (HN) and the fusion glycoprotein (F) mRNAs (N. Elango, J. E. Coligan, R. C. Jambou, and S. Venkatesan, J. Virol. 57: 481-489, 1986; M. K. Spriggs, R. A. Olmsted, S. Venkatesan, J. E. Coligan, and P. L. Collins, Virology 152:241-251, 1986). Synthetic oligonucleotides, designed from nucleotide sequences of the cDNAs, were used to direct dideoxynucleotide sequencing of gene junctions in PF3 genomic RNA (vRNA). From sequencing of vRNA, a sixth viral gene was detected and identified as the large nucleocapsid protein (L) gene by hybridization of a synthetic oligonucleotide to intracellular PF3 mRNAs separated by gel electrophoresis. The order of the six PF3 genes on vRNA was 3''-NP-P+C-M-F-HN-L-5''. The five intergenic regions consisted of the trinucleotide 3''-GAA. The PF3 genes initiated with semiconserved 10-nucleotide gene-start sequences and terminated with semiconserved 12-nucleotide gene-end sequences. The M gene terminated with an aberrant gene-end sequence; analysis of intracellular mRNA showed that this aberrant sequence correlated with a disproportionately high accumulation of readthrough mRNA. These studies showed that PF3 encodes six unique mRNAs (NP, P+C, M, F, HN, and L) that encode seven proteins (NP, P, C, M, F, HN, and L) and provided evidence of a close relationship between PF3 and Sendai (murine parainfluenza type 1) viruses.