Suspended particulate matter in indoor air: adjuvants and allergen carriers
Suspended particulate matter in indoor air: adjuvants and allergen carriers
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DOI：
10.1016/s0300-483x(00)00292-4
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发表时间：
2000-11-02
期刊：
影响因子：
4.5
通讯作者：
Ormstad, H
中科院分区：
文献类型：
--
作者：
Ormstad, H
这项研究的总体目的是调查空气中的室内灰尘颗粒如何导致过敏性免疫反应，从而导致哮喘和其他呼吸道症状。设定了以下目标：首先，量化和表征室内悬浮颗粒物 (SPM) 的数量、元素和尺寸分布；其次，确定 SPM 影响过敏性免疫反应的可能机制。奥斯陆家庭的 SPM 样本中的绝大多数颗粒直径都小于 2.5 微米。这种 PM2.5 组分除了含有大量的硫气溶胶和硅酸盐外，还含有大量的烟灰颗粒。其中大部分的直径都小于我妈妈。使用免疫金标记技术，我们发现这些烟灰颗粒的表面携带猫、狗和桦树过敏原。这些结果表明，室内SPM含有大量潜在的过敏原载体，即烟灰颗粒（碳聚集体），其中大多数直径小于1微米，因此能够将过敏原输送到呼吸树深处。我们进一步发现，柴油机尾气颗粒（DEP）很可能是SPM的主要烟灰成分，在体外吸附了几种众所周知的过敏原。此外，SPM 被发现可引起局部淋巴结炎症反应，并对卵清蛋白 (OA) 的 IgE 抗体的产生具有辅助活性。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
The overall purpose of this study was to investigate how airborne house dust particles may contribute to an allergic immune response, and thereby also to asthma and other respiratory symptoms. The following aims were set: first, to quantify and characterize indoor suspended particulate matter (SPM) with regard to amount, as well as elemental and size distribution, second, to identify possible mechanisms by which SPM may affect the allergic immune response. A vast majority of the particles in SPM samples from homes in Oslo were found to be less than 2.5 mum in diameter. This PM2.5 fraction contained, in addition to a large amount of sulfur aerosols and silicates, a lot of soot particles. Most of these were less than I mum in diameter. Using an immunogold labeling technique, we found that these soot particles carried cat, dog and birch allergens on their surface. These results show that indoor SPM contains a lot of potential allergen carriers, i.e. soot particles (carbon aggregates), most of them less that 1 mum in diameter and therefore able to transport allergens deep into the respiratory tree. We further found that diesel exhaust particles (DEP), which is likely the main soot component of SPM, adsorbed several well-known allergens in vitro. Furthermore, SPM was found to elicit a local lymph node inflammatory response, and to have an adjuvant activity on the production of IgE antibodies to ovalbumin (OA). (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.