Role of gene T in phage lambda tail assembly.
Role of gene T in phage lambda tail assembly.
复制标题
基因 T 在噬菌体 lambda 尾组装中的作用。
DOI：
10.1016/0042-6822(83)90200-3
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Hendrix,RW
中科院分区：
文献类型：
--
作者：
Tsui,LC;Hendrix,RW
我们研究了λ尾基因的热敏突变表型。λ Tts 40原噬菌体在非允许温度下的诱导和生长导致产生形态正常但生物学上无活性的尾，其可在提供基因T、U和Z产物的裂解物中被体外激活。因此，在尾装配途径中或在尾轴聚合完成后立即，基因V和U之间发生基因接触。λ Tts 40裂解物还含有λ相当数量的形态正常的噬菌体颗粒，其缺乏小尾蛋白gpH的切割。这一结果与以下事实一致，即9PH切割通常在组装途径中比geneTaction发现的时间更晚发生。在允许温度下产生的纯化λ Tts 40病毒体在gpH裂解方面正常。然而，它们比野生型病毒体对热灭活更敏感，这表明T基因产物存在于病毒体中。在允许的温度下，λ Tts 40突变蛋白即使在非允许的温度下合成，在体内也是有活性的。
We have studied the phenotype of a heat-sensitive mutation that defines λ tail gene Induction and growth at the nonpermissive temperature of a λTts40 prophage results in production of morphologically normal but biologically inactive tails, which can be activatedin vitroby lysates supplying the products of genesT,U, andZ. Thus, geneTacts between genesVandUin the tail assembly pathway or immediately after the completion of tail shaft polymerization. λTts40 lysates also contain λ substantial number of morphologically normal phage particles which are deficient in cleavage of the minor tail protein gpH. This result is consistent with the fact that 9PH cleavage normally occurs later in the assembly pathway than the time found for geneTaction. Purified λTts40 virions that were produced at the permissive temperature are normal with respect to gpH cleavage. However, they are more sensitive to heat inactivation than are wild-type virions, suggesting that theTgene product is in the virion. At the permissive temperature, the mutant protein made by λTts40 is activein vivoeven if it has been synthesized at the nonpermissive temperature.