Activation of yeast protein kinase C by Rho1 GTPase
Activation of yeast protein kinase C by Rho1 GTPase
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DOI：
10.1074/jbc.271.16.9193
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发表时间：
1996-04-19
影响因子：
4.8
通讯作者：
Levin, DE
中科院分区：
文献类型：
--
作者：
Kamada, Y;Qadota, H;Levin, DE
我们研究了芽殖酵母中必需的Rho1 GTP酶在细胞完整性信号中的作用。在蛋白激酶C(Pkc1)介导的细胞完整性信号通路中，条件性RO1突变体表现出与突变体相似的细胞裂解缺陷，而Pkc1的过表达抑制了Pkc1的过度表达。Rho1突变体在高温下的生长反应中也会受到途径激活的损害。Pkc1与Rho1在酵母抽提物中共沉淀，重组Rho1与Pkc1在体外以GTP依赖的方式结合。重组Rho1使Pkc1具有被磷脂酰丝氨酸刺激的能力。表示Rho1控制通过Pkc1的信号传输。
We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast. Conditional rho1 mutants display a cell lysis defect that is similar to that of mutants in the cell integrity signaling pathway mediated by protein kinase C (Pkc1), which is suppressed by overexpression of Pkc1. rho1 mutants are also impaired in pathway activation in response to growth at elevated temperature. Pkc1 co-immunoprecipitates with Rho1 in yeast extracts, and recombinant Rho1 associates with Pkc1 in vitro in a GTP-dependent manner. Recombinant Rho1 confers upon Pkc1 the ability to be stimulated by phosphatidylserine. indicating that Rho1 controls signal transmission through Pkc1.