Pd (core)-Au (shell) nanoparticles catalyzed conversion of NADH to NAD+ by UV-vis spectroscopy-A kinetic analysis
Pd (core)-Au (shell) nanoparticles catalyzed conversion of NADH to NAD+ by UV-vis spectroscopy-A kinetic analysis
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DOI：
10.1016/j.saa.2009.07.022
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发表时间：
2009-10-15
影响因子：
4.4
通讯作者：
Lee, K-P.
中科院分区：
文献类型：
--
作者：
Gopalan, A.;Ragupathy, D.;Lee, K-P.
用紫外可见光谱法研究了I'd（core）-Au（shell）纳米粒子（NPs）催化二氢烟酰胺腺嘌呤二核苷酸（NADH）转化为NAD（+）的动力学。采用微波辐射法制备了Pd（core）-Au（shell）纳米粒子。高分辨率透射电子显微镜图像揭示了核壳形态。X射线衍射图显示Pd和Au存在不同的晶畴。在不同的时间间隔内跟踪340 nm处的荧光强度的变化。在不同条件下测定NADH向NAD（+）的转化率。NADH转化为NAD（+）在较低浓度（高达0.04 mM）时为一级反应，超过0.04 mM时为伪一级反应。推导了Pd（核）Au-（壳）纳米粒子催化NADH转化为NAD（+）的速率常数。(C)2009 Elsevier B. V.保留所有权利。
Kinetics of I'd (core)-Au (shell) nanoparticles (NPs) catalyzed transformation of dihydronicotinamide adenine dinucleotide (NADH) to NAD(+) was monitored by UV-vis spectroscopy. Pd (core)-Au (shell) NPs were prepared by microwave irradiation method. High resolution transmission electron microscopy image reveals the core-shell morphology. X-ray diffraction pattern shows the presence of distinct crystalline domains for Pd and Au. The changes in absorbances at 340nm were followed for various time intervals. Rates of conversion of NADH to NAD(+) were determined for different conditions. The conversion of NADH to NAD(+) was to be first order with respect to NADH at lower concentrations (upto 0.04 mM) and pseudo-first-order beyond 0.04 mM. Rate constants for the Pd (core) Au-(shell) NPs catalyzed transformation of NADH to NAD(+) were deduced. (C) 2009 Elsevier B.V. All rights reserved.