Identification of Schlemm's Canal and Its Surrounding Tissues by Anterior Segment Fourier Domain Optical Coherence Tomography
Identification of Schlemm's Canal and Its Surrounding Tissues by Anterior Segment Fourier Domain Optical Coherence Tomography
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DOI：
10.1167/iovs.10-7009
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发表时间：
2011-08-01
影响因子：
4.4
通讯作者：
Araie, Makoto
中科院分区：
文献类型：
--
作者：
Usui, Tomohiko;Tomidokoro, Atsuo;Araie, Makoto
目的.应用眼前节傅立叶光学相干断层扫描（AS-FD-OCT）技术，结合组织学检查，对人眼Schlemm管（SC）和小梁网（TM）进行识别，并对活体人眼SC和TM进行定量评价。在摘除的人眼中，在注射粘弹性材料进行SC手术扩张之前和之后，通过AS-FD-OCT进行前房角成像，然后进行组织学检查。在60只活体人眼中，用Cohen的kappa值评价检查者之间识别SC的一致性，并在AS-FD-OCT图像的颞侧和鼻侧切片上测量SC和TM的长度以及TM的面积。在摘除的人眼中，在用高清晰度光栅扫描协议获得的AS-FD-OCT图像中观察到SC是薄的、线性的、透明的空间，但在用双角度径向扫描协议获得的图像中没有。这个空间在SC扩建后得到了扩大。在组织学研究中，证实SC与AS-FD-OCT图像中的位置相同。在活体人类受试者中可观察到的SC的kappa值为0.92或更高。SC的轴向长度平均为347.2 +/- 42.3 μ m，TM长度为466.9 +/- 60.7 μ m，TM面积为0.0671 +/- 0.0058 mm（2）。这些测量结果显示了足够的重复性和再现性。使用高清晰度图像的AS-FD-OCT，SC及其周围组织，成功地观察了在大多数的活眼，并定量评价在一个无创的方式。（Invest Ophthalmol维斯科学。2011;52：6934-6939）DOI：10.1167/iovs.10-7009
PURPOSE. To identify Schlemm's canal (SC) and trabecular meshwork (TM) by anterior segment Fourier-domain optical coherence tomography (AS-FD-OCT) with histologic confirmation in enucleated human eyes and to quantitatively evaluate SC and TM in living human eyes.METHODS. In enucleated human eyes, the imaging of the anterior chamber angle by AS-FD-OCT was performed before and after surgical expansion of SC with an injection of a viscoelastic material, followed by histologic examination. In 60 living human eyes, the agreement of identification of SC between examiners was evaluated with the Cohen's kappa values, and the lengths of SC and TM and the area of TM were measured on temporal and nasal sections of the AS-FD-OCT images.RESULTS. In enucleated human eyes, SC was observed to be a thin, linear, lucent space in the AS-FD-OCT image obtained with the high-definition raster scan protocol, but not in those obtained with the bi-angle radial scan protocol. This space was enlarged after the SC expansion. In the histologic study, the SC was confirmed to be in the same position as in the AS-FD-OCT images. The kappa values of observable SC in living human subjects were 0.92 or higher. The axial length of the SC averaged 347.2 +/- 42.3 mu m, TM length 466.9 +/- 60.7 mu m, and TM area 0.0671 +/- 0.0058 mm(2). These measurements showed sufficient repeatability and reproducibility.CONCLUSIONS. Using the high-definition images of the AS-FD-OCT, SC and its surrounding tissues were successfully observed in most of the living eyes and were quantitatively evaluated in a noninvasive manner. (Invest Ophthalmol Vis Sci. 2011;52:6934-6939) DOI:10.1167/iovs.10-7009