Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.
Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.
复制标题
大肠杆菌个体核糖体 RNA 转录单位中间隔 tRNA 基因的鉴定。
DOI：
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发表时间：
1977
影响因子：
11.1
通讯作者：
M. Nomura
中科院分区：
文献类型：
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作者：
E. A. Morgan;T. Ikemura;M. Nomura
转移RNA基因（“间隔区tRNA基因”）存在于大肠杆菌16S和23S rRNA基因之间的间隔区。我们分析了7个不同染色体位置的rRNA操纵子携带的间隔tRNA基因。其中6个在质粒上分离，1个在转导噬菌体上分离。我们发现，除了之前鉴定的tRNA2Glu和tRNAIIle两个基因外，还有一个间隔tRNA基因编码tRNAIBAla。在研究的7个rRNA操纵子中，3个同时具有tRNAIBAla和tRNAIIle基因，其余4个在间隔中具有tRNA2Glu基因。此外，tRNAIAsp基因在两个不同的rRNA操纵子的远端附近被发现。
Transfer RNA genes ("spacer tRNA genes") are present in the spacer region between 16S and 23S rRNA genes in Escherichia coli. We have analyzed spacer tRNA genes carried by seven rRNA operons with different chromosomal locations. Six of these were isolated on plasmids and one on a transducing phage. We found that, in addition to the two previously identified genes for tRNA2Glu and tRNAIIle, there is a spacer tRNA gene which codes for tRNAIBAla. Of the seven rRNA operons studied, three had both tRNAIBAla and tRNAIIle genes, and the remaining four had the tRNA2Glu gene in their spacers. In addition, genes for tRNAIAsp were found near the distal ends of two different rRNA operons.