Anti-inflammatory properties of narrow-band blue light.
Anti-inflammatory properties of narrow-band blue light.
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窄带蓝光的抗炎特性。
DOI：
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发表时间：
2006
影响因子：
1.5
通讯作者：
W. Lee
中科院分区：
文献类型：
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作者：
Elaine Shnitkind;E. Yaping;S. Geen;A. Shalita;W. Lee
背景
窄带蓝光（420 nm）已被证明在治疗寻常痤疮中的安全性和有效性。其通过对痤疮丙酸杆菌的血红素代谢表现出光毒性作用而起作用。先前使用蓝光的研究显示，炎症病变的改善程度高于粉刺，未经治疗的一侧也有一些改善。细胞因子已被证明在炎症的发展中起关键作用。促炎细胞因子如IL-1 α的表达已显示导致血管和真皮粘附分子的表达、炎性细胞的化学吸引和其他炎性介质的刺激。此外，UVB辐射作为细胞介导的免疫反应的有效调节剂。
目的
本研究使用IL-1 α和ICAM-1作为炎症标志物，研究了窄带蓝光在存在和不存在细胞因子和UVB的情况下对炎症过程的影响。
方法
两个永生化的角质形成细胞系进行了比较：HaCaT，自发永生化的基因改变的细胞系，和hTERT，通过稳定转染的原代细胞培养与人端粒酶逆转录酶。用INF-γ和TNF-α处理细胞，并暴露于UVB（312 nm，50 mJ/cm 2）和/或蓝光（420 nm，54 mJ/cm 2和134 mJ/cm 2）。ELISA定量检测IL-1 α和ICAM-1的表达。
结果
结果显示，蓝光和低剂量UVB处理HaCaT和hTERT细胞可抑制苦参碱诱导的IL-1 α的产生。当暴露于蓝光时，HaCaT细胞中IL-1 α的水平降低了82%，hTERT细胞中降低了75%。当蓝光与UVB结合使用时，HaCaT细胞和hTERT细胞中的凋亡率分别降低了95%和91%。ICAM-1表达在HaCaT中也同样减少，但在hTERT细胞中没有。
结论
这项研究表明，窄带蓝光通过减少姜黄素诱导的IL-1 α和ICAM-1的产生对角质形成细胞具有抗炎作用。此外，蓝光表现出与低剂量UVB光的协同效应。这些结果扩展了窄带蓝光在调节炎症过程中的特性，并将有助于测试其在不同炎症皮肤条件下的光治疗应用。
BACKGROUND
Narrow-band blue light (420 nm) has demonstrated safety and efficacy in the treatment of acne vulgaris. It works by exhibiting a phototoxic effect on the heme metabolism of Propionibacterium acnes. Previous studies using blue light showed more improvement in inflammatory lesions than in comedones, as well as some improvement on the untreated side. Cytokines have demonstrated a critical role in the development of inflammation. The expression of pro-inflammatory cytokines such as IL-1alpha have been shown to result in the expression of vascular and dermal adhesion molecules, the chemoattraction of inflammatory cells, and the stimulation of other inflammatory mediators. In addition, UVB radiation serves as a potent modulator of cell-mediated immune responses.
PURPOSE
This study investigated the effect of narrow-band blue light on the inflammatory process in the presence and absence of cytokines and UVB using IL-1alpha and ICAM-1 as markers for inflammation.
METHODS
Two immortalized keratinocyte cell lines were compared: HaCaT, produced by spontaneous immortalization of a genetically altered cell line, and hTERT, obtained by stable transfection of primary cell culture with human telomerase reverse transcriptase. Cells were treated with INF-y and TNF-alpha and exposed to UVB (312 nm at 50 mJ/cm2) and/or blue light (420 nm at 54 mJ/cm2 and 134 mJ/cm2). The expression of IL-1alpha and ICAM-1 was measured by quantitative ELISA.
RESULTS
The results showed that blue light and low-dose UVB treatment of HaCaT and hTERT cells resulted in inhibition of cytokine-induced production of IL-1alpha. The level of IL-1alpha decreased by 82% in HaCaT and by 75% in hTERT cells when exposed to blue light. It decreased by 95% in HaCaT and by 91% in hTERT cells when blue light was used in combination with UVB. ICAM-1 expression was similarly reduced in HaCaT, but not in hTERT cells.
CONCLUSIONS
This study showed that narrow-band blue light has anti-inflammatory effects on keratinocytes by decreasing the cytokine-induced production of IL-1alpha and ICAM-1. In addition, blue light demonstrated synergistic effects with low-dose UVB light. These results expand the properties of narrow-band blue light in modulating the inflammatory process and will facilitate testing of its phototherapeutic applications in different inflammatory skin conditions.