QUANTITATIVE AUTORADIOGRAPHY OF BETA-1-ADRENERGIC AND BETA-2-ADRENERGIC RECEPTORS IN RAT-BRAIN
QUANTITATIVE AUTORADIOGRAPHY OF BETA-1-ADRENERGIC AND BETA-2-ADRENERGIC RECEPTORS IN RAT-BRAIN
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DOI：
10.1073/pnas.81.5.1585
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
WOLFE, BB
中科院分区：
文献类型：
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作者：
RAINBOW, TC;PARSONS, B;WOLFE, BB
采用定量放射自显影技术对大鼠脑内β进行定位。1和。2-adrenergic受体。这些受体在体外用125i标记的pindolol（一种β的拮抗剂）标记。-肾上腺素能受体非选择性地结合。1和。2亚型。125i标记品多洛尔选择性抑制β特异性拮抗剂结合。1和。2个受体允许。β的可视化。-肾上腺素能受体亚型高水平的β。1个受体分布于扣带皮层、大脑皮层第1层和第2层、海马、Calleja岛和丘脑明胶核、中背核和腹侧核。高水平的β。2个受体位于小脑分子层、硬脑膜上方、丘脑中央核、室旁核和尾侧外侧核。大致相等的。β水平。1和。2个受体分布于黑质、嗅结节、大脑皮层第四层、内侧视前核和髓质所有核。的比值的显著差异。1到。。2受体在脑区的分布表明。-肾上腺素能受体可能在神经元功能中发挥不同的作用。
Quantitative autoradiography was used to localize in rat brain .beta.1- and .beta.2-adrenergic receptors. These receptors were labeled in vitro with 125I-labeled pindolol, an antagonist of .beta.-adrenergic receptors that binds nonselectively to both .beta.1 and .beta.2 subtypes. The selective inhibition of 125I-labeled pindolol binding with specific antagonists of .beta.1 and .beta.2 receptors allowed the visualization of .beta.-adrenergic receptor subtypes. High levels of .beta.1 receptors were observed in the cingulate cortex, layers I and II of the cerebral cortex, the hippocampus, the Islands of Calleja and the gelatinosus, mediodorsal, and ventral nuclei of the thalamus. High levels of .beta.2 receptors were found in the molecular layer of the cerebellum, over pia mater and in the central, paraventricular, and caudal lateral posterior thalamic nuclei. Approximately equal levels of .beta.1 and .beta.2 receptors occurred in the substantia nigra, the olfactory tubercle, layer IV of the cerebral cortex, the medial preoptic nucleus and all nuclei of the medulla. The pronounced differences in the ratio of .beta.1 to .beta.2 receptors among brain regions suggests that the subtypes of .beta.-adrenergic receptors may play different roles in neuronal function.