Comparative studies with EPR and MRI on the in vivo tissue redox status estimation using redox-sensitive nitroxyl probes: influence of the choice of the region of interest.
Comparative studies with EPR and MRI on the in vivo tissue redox status estimation using redox-sensitive nitroxyl probes: influence of the choice of the region of interest.
复制标题
DOI：
10.1080/10715762.2018.1427235
复制
发表时间：
2018-03
影响因子：
3.3
通讯作者：
Krishna MC
中科院分区：
文献类型：
--
作者：
Matsumoto KI;Mitchell JB;Krishna MC
通过MR氧化还原成像（MRRI）技术估计硝酰基造影剂的体内衰减率，并与通过电子顺磁共振光谱（EPRS）和成像（EPRI）获得的衰减率进行比较。MRRI是一种采用T1加权脉冲序列的动态成像技术，可以通过T1加权对比度可视化硝酰诱导的信号强度增强。EPR技术可以直接测量顺磁性硝酰基自由基。用T1加权SPGR序列在4.7T下扫描雌性C3 H小鼠的SCC荷瘤和正常腿，以硝酰基自由基，氨甲酰基-PROXYL（CmP）作为对比剂。同样，正常肌肉和肿瘤组织中的CmP的时间过程中获得使用700 MHz EPR光谱仪与表面线圈。用300 MHz CW EPR成像仪对CmP进行时程成像。与MRRI相比，EPRS和EPRI给出的CmP衰减速率较慢。在MRRI获得的图像中发现肿瘤组织周边区域的相对较慢的衰减速率可能有助于EPRS和/或EPRI测量观察到的较慢的衰减速率。为了可靠地确定组织的氧化还原状态从还原率的硝酰基，如CmP，在肿瘤组织中的异质结构必须考虑。T1加权MRI的高空间和时间分辨率以及硝酰基的T1增强能力支持使用该方法来映射组织氧化还原状态，其可以是基于肿瘤微环境指导适当治疗的有用生物标志物。
In vivo decay rates of a nitroxyl contrast agent were estimated by a MR redox imaging (MRRI) technique and compared with the decay rates obtained by the electron paramagnetic resonance spectroscopy (EPRS) and imaging (EPRI). MRRI is a dynamic imaging technique employing T1-weighted pulse sequence, which can visualize a nitroxyl-induced enhancement of signal intensity by T1-weighted contrast. EPR techniques can directly measure the paramagnetic nitroxyl radical. Both the SCC tumor-bearing and normal legs of a female C3H mouse were scanned by T1-weighted SPGR sequence at 4.7 T with the nitroxyl radical, carbamoyl-PROXYL (CmP), as the contrast agent. Similarly, the time course of CmP in normal muscle and tumor tissues was obtained using an 700 MHz EPR spectrometer with a surface coil. The time course imaging of CmP was also performed by 300 MHz CW EPR imager. EPRS and EPRI gave slower decay rates of CmP compared to the MRRI. Relatively slow decay rate at peripheral region of the tumor tissues, which was found in the image obtained by MRRI, may contribute to the slower decay rates observed by EPRS and/or the EPRI measurements. To reliably determine the tissue redox status from the reduction rates of nitroxyls such as CmP, heterogenic structure in the tumor tissue must be considered. The high spatial and temporal resolution of T1-weighted MRI and the T1-enhancing capabilities of nitroxyls support the use of this method to map tissue redox status which can be a useful biomarker to guide appropriate treatments based on the tumor microenvironment.
登录
查看更多内容
影响因子：
11.5
作者：
Matsumoto, K;Hyodo, F;Krishna, MC
通讯作者：
Krishna, MC
影响因子：
3.3
作者：
Ilangovan, G;Li, HQ;Kuppusamy, P
通讯作者：
Kuppusamy, P
影响因子：
6.7
作者：
GRIFFETH, LK;ROSEN, GM;DRAYER, BP
通讯作者：
DRAYER, BP
影响因子：
6.5
作者：
Takeshita, K;Takajo, T;Utsumi, H
通讯作者：
Utsumi, H
影响因子：
11.2
作者：
Takeshita K;Kawaguchi K;Fujii-Aikawa K;Ueno M;Okazaki S;Ono M;Krishna MC;Kuppusamy P;Ozawa T;Ikota N
通讯作者：
Ikota N